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dc.contributor.authorEdema, Hilary
dc.contributor.authorAshraf, Muhammad Furqan
dc.contributor.authorSamkumar, Amos
dc.contributor.authorJaakola, Laura Elina
dc.contributor.authorKarppinen, Katja Hannele
dc.date.accessioned2024-10-03T10:55:27Z
dc.date.available2024-10-03T10:55:27Z
dc.date.issued2024-07-14
dc.description.abstractCellulose is a major renewable resource for a wide variety of sustainable industrial products. However, for its utilization, finding new efficient enzymes for plant cell wall depolymerization is crucial. In addition to microbial sources, cellulases also exist in plants, however, are less studied. Fleshy fruit ripening includes enzymatic cell wall hydrolysis, leading to tissue softening. Therefore, bilberry (Vaccinium myrtillus L.), which produces small fruits that undergo extensive and rapid softening, was selected to explore cellulases of plant origin. We identified 20 glycoside hydrolase family 9 (GH9) cellulases from a recently sequenced bilberry genome, including four of which showed fruit ripening-specific expression and could be associated with fruit softening based on phylogenetic, transcriptomic and gene expression analyses. These four cellulases were secreted enzymes: two B-types and two C-types with a carbohydrate binding module 49. For functional characterization, these four cellulases were expressed in Pichia pastoris. All recombinant enzymes demonstrated glucanase activity toward cellulose and hemicellulose substrates. Particularly, VmGH9C1 demonstrated high activity and ability to degrade cellulose, xyloglucan, and glucomannan. In addition, all the enzymes retained activity under wide pH (6–10) and temperature ranges (optimum 70 °C), revealing the potential applications of plant GH9 cellulases in the industrial bioprocessing of lignocellulose.en_US
dc.identifier.citationEdema, Ashraf, Samkumar, Jaakola, Karppinen. Characterization of cellulases from softening fruit for enzymatic depolymerization of cellulose. Carbohydrate Polymers. 2024;343en_US
dc.identifier.cristinIDFRIDAID 2290379
dc.identifier.doi10.1016/j.carbpol.2024.122493
dc.identifier.issn0144-8617
dc.identifier.issn1879-1344
dc.identifier.urihttps://hdl.handle.net/10037/35019
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalCarbohydrate Polymers
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2024 The Author(s)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.titleCharacterization of cellulases from softening fruit for enzymatic depolymerization of celluloseen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)