Modulation of cAMP levels by β-stimulation and PDE3 inhibition during hypothermia. An in vitro study on embryonic cardiac cells from rat
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https://hdl.handle.net/10037/37076Date
2022-05-15Type
Master thesisMastergradsoppgave
Author
Furuholmen, MarkusAbstract
Methods: Embryonic cardiac cells were incubated at 37, 30 and 20 °C. Intracellular levels of cAMP were quantified with LC-MS/MS in control cells and after exposure with milrinone and isoprenaline after 3, 15 and 30 minutes.
Results: Significant elevation of cAMP was observed after incubation with 100 μM and 1 mM milrinone at all temperatures (p<0.05). Similarly, isoprenaline induced significant cAMP elevations at all temperatures, but only in a concentration of 10 μM (p<0.05). Incubating control cells, not exposed to any drug, resulted in significant cAMP elevation after 3 and 15 minutes during moderate hypothermia (30 °C) compared to normothermia (p<0.05). Corresponding cAMP elevation was not present during severe hypothermia (20 °C).
Conclusion: The present findings demonstrate that milrinone and isoprenaline elevates cAMP at temperatures down to 20 °C. In contrast, the relation between basal levels of cAMP and temperature seems non-linear, as only moderate hypothermia induces a significant elevation of cAMP compared to normothermia.
Publisher
UiT Norges arktiske universitetUiT The Arctic University of Norway
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