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dc.contributor.advisorPerander, Maria
dc.contributor.advisorKnutsen, Erik
dc.contributor.advisorJohansen, Steinar
dc.contributor.authorBrun, Elsebeth Sophie
dc.date.accessioned2013-02-25T08:33:21Z
dc.date.available2013-02-25T08:33:21Z
dc.date.issued2012-12-17
dc.description.abstractLong non-coding RNAs (lncRNAs) are involved in several important biological processes, and several lncRNAs have been associated with human diseases, particularly cancer. LncRNAs regulate gene expression at different levels. LncRNAs exert their functions through interactions with DNA, RNA or proteins. In this study, we established a method for investigating interactions between lncRNAs and protein complexes in human breast cancer cell lines. We used the well-known interaction between HOTAIR and the PRC2 member Suz12 to work out a RNA immunoprecipitation protocol in the lab, using an antibody targeting Suz12 to co-immunoprecipitate HOTAIR from nuclear lysates from the Hs578 breast cancer cells. We achieved a significant enrichment of HOTAIR in the immunoprecipitate from nuclear extracts from Hs578T. Differential expression of HOTAIR in the three breast cancer cell lines Hs578T, MDA-MB-231 and MCF-7 were also observed.en
dc.identifier.urihttps://hdl.handle.net/10037/4847
dc.identifier.urnURN:NBN:no-uit_munin_4557
dc.language.isoengen
dc.publisherUniversitetet i Tromsøen
dc.publisherUniversity of Tromsøen
dc.rights.accessRightsopenAccess
dc.rights.holderCopyright 2012 The Author(s)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/3.0en_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)en_US
dc.subject.courseIDMBI-3941en
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762en
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762en
dc.subjectLong non-coding RNAsen
dc.subjectRNA immunoprecipitationen
dc.subjectBreast canceren
dc.titleEstablishing a method for RNA immunoprecipitation to study interactions between long non-coding RNAs and proteinsen
dc.typeMaster thesisen
dc.typeMastergradsoppgaveen


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Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)