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dc.contributor.authorJohnsen, Hanne Risan
dc.contributor.authorKrause, Kirsten
dc.date.accessioned2014-02-18T13:45:25Z
dc.date.available2014-02-18T13:45:25Z
dc.date.issued2014
dc.description.abstractReliable, rapid and inexpensive detection of cellulolytic enzymes that can be used for a wide variety of biological and environmental samples are currently in high demand. Here, a new cellulase detection protocol is described that circumvents problems observed with popular agar-based methods by exploiting the ability of carboxymethylcellulose (CMC) to form gel-like surfaces on its own. These pure CMC-layers are sensitive to cellulolytic degradation and stainable by Gram’s iodine without showing unwelcome reactions with other enzymes. The staining intensity negatively correlates with the enzyme activity and can be used for quantification. Cellulase activities are not obstructed by high sugar contents (e.g., in plant material) which limit the applicability of other quantification methods, making our new method particularly attractive for screening of plant extracts. A useful variant of this new method is its applicability to plant tissue prints for spatial mapping of the cellulolytic activity in a zymogram-like fashion.en
dc.identifier.citationInternational Journal of Molecular Sciences 15(2014) nr. 1 s. 830-838en
dc.identifier.cristinIDFRIDAID 1097681
dc.identifier.doihttp://dx.doi.org/10.3390/ijms15010830
dc.identifier.issn1422-0067
dc.identifier.urihttps://hdl.handle.net/10037/5846
dc.identifier.urnURN:NBN:no-uit_munin_5540
dc.language.isoengen
dc.publisherMDPIen
dc.rights.accessRightsopenAccess
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480::Plant physiology: 492en
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Plantefysiologi: 492en
dc.titleCellulase activity screening using pure carboxymethylcellulose: Application to soluble cellulolytic samples and to plant tissue prints.en
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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