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dc.contributor.authorLarsen, Anett Kristin
dc.contributor.authorNymo, Ingebjørg Helena
dc.contributor.authorBriquemont, Benjamin
dc.contributor.authorSørensen, Karen Kristine
dc.contributor.authorGodfroid, Jacques
dc.date.accessioned2014-02-18T13:48:20Z
dc.date.available2014-02-18T13:48:20Z
dc.date.issued2013
dc.description.abstractMarine mammal Brucella spp. have been isolated from pinnipeds (B. pinnipedialis) and cetaceans (B. ceti) from around the world. Although the zoonotic potential of marine mammal brucellae is largely unknown, reports of human disease exist. There are few studies of the mechanisms of bacterial intracellular invasion and multiplication involving the marine mammal Brucella spp. We examined the infective capacity of two genetically different B. pinnipedialis strains (reference strain; NTCT 12890 and a hooded seal isolate; B17) by measuring the ability of the bacteria to enter and replicate in cultured phagocytes and epithelial cells. Human macrophage-like cells (THP-1), two murine macrophage cell lines (RAW264.7 and J774A.1), and a human malignant epithelial cell line (HeLa S3) were challenged with bacteria in a gentamicin protection assay. Our results show that B. pinnipedialis is internalized, but is then gradually eliminated during the next 72 – 96 hours. Confocal microscopy revealed that intracellular B. pinnipedialis hooded seal strain colocalized with lysosomal compartments at 1.5 and 24 hours after infection. Intracellular presence of B. pinnipedialis hooded seal strain was verified by transmission electron microscopy. By using a cholesterol-scavenging lipid inhibitor, entrance of B. pinnipedialis hooded seal strain in human macrophages was significantly reduced by 65.8 % (± 17.3), suggesting involvement of lipid-rafts in intracellular entry. Murine macrophages invaded by B. pinnipedialis do not release nitric oxide (NO) and intracellular bacterial presence does not induce cell death. In summary, B. pinnipedialis hooded seal strain can enter human and murine macrophages, as well as human epithelial cells. Intracellular entry of B. pinnipedialis hooded seal strain involves, but seems not to be limited to, lipid-rafts in human macrophages. Brucella pinnipedialis does not multiply or survive for prolonged periods intracellulary.en
dc.identifier.citationPLoS ONE (2013), vol. 8(12): e84861.en
dc.identifier.cristinIDFRIDAID 1080968
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0084861
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/10037/5847
dc.identifier.urnURN:NBN:no-uit_munin_5547
dc.language.isoengen
dc.publisherPublic Library of Science (PLoS)en
dc.rights.accessRightsopenAccess
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::General immunology: 478en
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell immunologi: 478en
dc.titleEntrance and survival of Brucella pinnipedialis hooded seal strain in human macrophages and epithelial cellsen
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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