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dc.contributor.authorIliev, Dimitar Borisov
dc.contributor.authorSkjÆveland, ingrid
dc.contributor.authorJørgensen, Jorunn B
dc.date.accessioned2014-02-18T14:05:49Z
dc.date.available2014-02-18T14:05:49Z
dc.date.issued2013
dc.description.abstractBackground: Bacterial DNA is well-known for its potent immunostimulatory properties which have been attributed to the abundance of CpG dinucleotides within the genomes of prokaryotes. Research has found that mammalian TLR9 is a receptor which mediates the immune response to CpG DNA; however, its functional properties in non-mammalian vertebrates are still poorly characterized. Leukocytes isolated from lower vertebrates, including teleosts, respond to CpG DNA and TLR9 has been identified in many fish species; however, the ligand-binding properties of fish TLR9 have, so far, not been studied. The fact that some vertebrates, like chicken, lack TLR9 and use an alternative molecule (TLR21) as a receptor for CpGs has questioned the functional conservation of TLR9 within vertebrates. Results: In the current study, TLR9 from Atlantic salmon (SsTLR9) has been found to interact with synthetic oligonucleotides via a CpG-independent but a pH-dependent mechanism. The endogenous receptor, expressed by primary mononuclear phagocytes colocalizes with CpG oligonucleotides (ODNs) in vesicles that appear to be endosomes. When overexpressed in salmonid cell lines, SsTLR9 spontaneously activates ISRE-containing promoters of genes involved in the IFN response; however, the transgenic receptor fails to translocate to CpG-containing endosomes. This indicates that only specific immune cell types have the ability to relocate the receptor to the appropriate cellular compartments where it may become activated by its ligand. In addition, through co-precipitation and mass spectrometry, two salmon proteins - hnRNPA0 and NCOA5, which both contain RNA-binding domains (RRM), were found to bind CpG ODNs, suggesting they may be involved in the CpG response in salmon leukocytes. Conclusion: The presented data are the first to demonstrate that the DNA-binding properties of TLR9 are conserved between teleosts and mammals. The current study also identifies additional molecules which may function as mediators of the immunostimulatory properties of foreign DNA.en
dc.identifier.citationBMC Immunology 14:12(2013)en
dc.identifier.cristinIDFRIDAID 1032893
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2172-14-12
dc.identifier.issn1471-2172
dc.identifier.urihttps://hdl.handle.net/10037/5853
dc.identifier.urnURN:NBN:no-uit_munin_5548
dc.language.isoengen
dc.publisherBioMed Centralen
dc.rights.accessRightsopenAccess
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::General immunology: 478en
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell immunologi: 478en
dc.titleCpG oligonucleotides bind TLR9 and RRM-Containing proteins in Atlantic Salmon (Salmo salar)en
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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