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dc.contributor.authorScuda, Nelly
dc.contributor.authorMadinda, Nadege
dc.contributor.authorAkoua-Koffi, Chantal
dc.contributor.authorAdjogoua, Edgard V
dc.contributor.authorWevers, Diana
dc.contributor.authorHofmann, Jörg
dc.contributor.authorCameron, Kenneth M.
dc.contributor.authorLeendertz, Siv Aina Jensen
dc.contributor.authorCouacy-Hymann, Emmanuel
dc.contributor.authorRobbins, Martha M.
dc.contributor.authorBoesch, Christophe
dc.contributor.authorJarvis, Michael
dc.contributor.authorMoens, Ugo
dc.contributor.authorCalvignac-Spencer, Sébastien
dc.contributor.authorLeendertz, Fabian H
dc.contributor.authorEhlers, Bernhard
dc.contributor.authorEhlers, B
dc.date.accessioned2014-03-21T07:17:08Z
dc.date.available2014-03-21T07:17:08Z
dc.date.issued2013
dc.description.abstractPolyomaviruses are a family of small non-enveloped DNA viruses that encode oncogenes and have been associated, to greater or lesser extent, with human disease and cancer. Currently, twelve polyomaviruses are known to circulate within the human population. To further examine the diversity of human polyomaviruses, we have utilized a combinatorial approach comprised of initial degenerate primer-based PCR identification and phylogenetic analysis of nonhuman primate (NHP) polyomavirus species, followed by polyomavirus-specific serological analysis of human sera. Using this approach we identified twenty novel NHP polyomaviruses: nine in great apes (six in chimpanzees, two in gorillas and one in orangutan), five in Old World monkeys and six in New World monkeys. Phylogenetic analysis indicated that only four of the nine chimpanzee polyomaviruses (six novel and three previously identified) had known close human counterparts. To determine whether the remaining chimpanzee polyomaviruses had potential human counterparts, the major viral capsid proteins (VP1) of four chimpanzee polyomaviruses were expressed in E. coli for use as antigens in enzyme-linked immunoassay (ELISA). Human serum/plasma samples from both Coˆ te d’Ivoire and Germany showed frequent seropositivity for the four viruses. Antibody pre-adsorption-based ELISA excluded the possibility that reactivities resulted from binding to known human polyomaviruses. Together, these results support the existence of additional polyomaviruses circulating within the human population that are genetically and serologically related to existing chimpanzee polyomaviruses.en
dc.identifier.citationPLoS Pathogens (2013), vol. 9(6): e1003429.en
dc.identifier.cristinIDFRIDAID 1038016
dc.identifier.doihttps://doi.org/10.1371/journal.ppat.1003429
dc.identifier.issn1553-7366
dc.identifier.urihttps://hdl.handle.net/10037/6029
dc.identifier.urnURN:NBN:no-uit_munin_5750
dc.language.isoengen
dc.publisherPublic Library of Science (PLoS)en
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical genetics: 714en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk genetikk: 714en
dc.titleNovel Polyomaviruses of Nonhuman Primates: Genetic and Serological Predictors for the Existence of Multiple Unknown Polyomaviruses within the Human Populationen
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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