Abstract
The aim of this project was to optimize the extraction of esterase enzymes from undifferentiated herring rest raw material. This was conducted by comparing, in laboratory scale, the outcome of using homogenised and non-homogenised raw material, and by extracting with buffers of different pH. Further on, clarification experiments of flocculation were performed on the optimal extract. The focus was to define methods enabling a large-scale production, with regards to one of the esterases found, and also determined in a previous project, to be of particular commercial interest.