Show simple item record

dc.contributor.advisorSager, Georg
dc.contributor.authorRafiq, Sarah
dc.date.accessioned2015-03-09T14:16:27Z
dc.date.available2015-03-09T14:16:27Z
dc.date.issued2015-01-27
dc.description.abstractThe transport of cyclic nucleotides out of the cell is energy requiring, dependent on ATP- hydrolysis. The ability to stimulate this ATPase is a hallmark of substrates for ABC-transporters e.g. ABCC5, ABCC4. It is believed that ABCC5 transporter has selective high affinity for cGMP. Previous studies support the idea that ABCC5 contribute to cGMP transport by human erythrocytes. Human erythrocyte membrane possesses cGMP transport system that utilizes ATP for its activity. Present study was conducted to measure, “Cyclic nucleotide dependent ATPase activity in inside out vesicles from human erythrocytes” by an In-house assay method and to compare it with commercially available kit to make cost benefit analysis. The main findings were: 1) The inorganic phosphate standard curves were linear for relevant biological concentrations, observed for both in-house and commercial assay. 2) The membrane protein (i.e. IOV) concentrations raised the inorganic phosphate concentrations linearly. 3) It was possible to distinguish cGMP-stimulated activity from basal ATPase activity in hRBC IOV, even though the difference was small. 4) The two phosphate assays (in-house and commercial) had both advantages and disadvantages, none being superior to the other one.en_US
dc.identifier.urihttps://hdl.handle.net/10037/7379
dc.identifier.urnURN:NBN:no-uit_munin_6965
dc.language.isoengen_US
dc.publisherUiT Norges arktiske universiteten_US
dc.publisherUiT The Arctic University of Norwayen_US
dc.rights.accessRightsopenAccess
dc.rights.holderCopyright 2015 The Author(s)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/3.0en_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)en_US
dc.subject.courseIDMBI-3910en_US
dc.subjectMedical Biologyen_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk biokjemi: 726en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical biochemistry: 726en_US
dc.titleCyclic nucleotide dependent ATPase activity in inside out vesicles from human erythrocytesen_US
dc.typeMaster thesisen_US
dc.typeMastergradsoppgaveen_US


File(s) in this item

Thumbnail
Thumbnail

This item appears in the following collection(s)

Show simple item record

Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)