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dc.contributor.authorHolderbaum, Daniel Ferreira
dc.contributor.authorCuhra, Marek
dc.contributor.authorWickson, Fern
dc.contributor.authorOrth, Afonso Inácio
dc.contributor.authorNodari, Rubens Onofre
dc.contributor.authorBøhn, Thomas
dc.date.accessioned2016-03-03T09:26:44Z
dc.date.available2016-03-03T09:26:44Z
dc.date.issued2015-08-11
dc.description.abstractInsect resistance is the second most common trait globally in cultivated genetically modified (GM) plants. Resistance is usually obtained by introducing into the plant’s genome genes from the bacterium Bacillus thuringiensis (Bt) coding for insecticidal proteins (Cry proteins or toxins) that target insect pests. The aim of this study was to examine the hypothesis that a chronic, high-dose dietary exposure to leaves of a Bt–maize hybrid (GM event MON810, expressing a transgenic or recombinant Cry1Ab toxin), exerted no adverse effects on fitness parameters of the aquatic nontarget organism Daphnia magna (water flea) when compared to an identical control diet based on leaves of the non-GM near-isoline. Cry1Ab was immunologically detected and quantified in GM maize leaf material used for Daphnia feed. A 69-kD protein near Bt’s active core-toxin size and a 34-kD protein were identified. The D. magna bioassay showed a resource allocation to production of resting eggs and early fecundity in D. magna fed GM maize, with adverse effects for body size and fecundity later in life. This is the first study to examine GM-plant leaf material in the D. magna model, and provides of negative fitness effects of a MON810 maize hybrid in a nontarget model organism under chronic, high dietary exposure. Based upon these results, it is postulated that the observed transgenic proteins exert a nontarget effect in D. magna and/or unintended changes were produced in the maize genome/metabolome by the transformation process, producing a nutritional difference between GM-maize and non-GM near-isoline.en_US
dc.descriptionPublished version. Source at <a href=http://doi.org/10.1080/15287394.2015.1037877>http://doi.org/10.1080/15287394.2015.1037877</a>.en_US
dc.identifier.citationJournal of Toxicology and Environmental Health 2015, 78(15):993-1007en_US
dc.identifier.cristinIDFRIDAID 1286152
dc.identifier.doi10.1080/15287394.2015.1037877
dc.identifier.issn1528-7394
dc.identifier.urihttps://hdl.handle.net/10037/8627
dc.identifier.urnURN:NBN:no-uit_munin_8180
dc.language.isoengen_US
dc.publisherTaylor & Francisen_US
dc.rights.accessRightsopenAccess
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Plantefysiologi: 492en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480::Plant physiology: 492en_US
dc.titleChronic Responses of Daphnia magna under Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt-Maize Hybrid and its Conventional Near-Isolineen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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