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dc.contributor.authorCarayol, Marion
dc.contributor.authorLicaj, Idlir
dc.contributor.authorAchaintre, David
dc.contributor.authorVineis, Paolo
dc.contributor.authorKey, Timothy J.
dc.contributor.authorMoret, N. Charlotte Onland
dc.contributor.authorScalbert, Augustin
dc.contributor.authorRinaldi, Sabina
dc.contributor.authorFerrari, Pietro
dc.date.accessioned2016-03-03T10:32:28Z
dc.date.available2016-03-03T10:32:28Z
dc.date.issued2015-08-14
dc.description.abstractObjective Although metabolic profiles have been associated with chronic disease risk, lack of temporal stability of metabolite levels could limit their use in epidemiological investigations. The present study aims to evaluate the reliability over a two-year period of 158 metabolites and compare reliability over time in fasting and non-fasting serum samples. <p>Methods Metabolites were measured with the AbsolueIDQp180 kit (Biocrates, Innsbruck, Austria) by mass spectrometry and included acylcarnitines, amino acids, biogenic amines, hexoses, phosphatidylcholines and sphingomyelins. Measurements were performed on repeat serum samples collected two years apart in 27 fasting men from Turin, Italy, and 39 nonfasting women from Utrecht, The Netherlands, all participating in the European Prospective Investigation into Cancer and Nutrition (EPIC) study. Reproducibility was assessed by estimating intraclass correlation coefficients (ICCs) in multivariable mixed models. <p>Results In fasting samples, a median ICC of 0.70 was observed. ICC values were <0.50 for 48% of amino acids, 27% of acylcarnitines, 18% of lysophosphatidylcholines and 4% of phosphatidylcholines. In non-fasting samples, the median ICC was 0.54. ICC values were <0.50 for 71% of acylcarnitines, 48% of amino acids, 44% of biogenic amines, 36% of sphingomyelins, 34% of phosphatidylcholines and 33% of lysophosphatidylcholines. Overall, reproducibility was lower in non-fasting as compared to fasting samples, with a statistically significant difference for 19–36% of acylcarnitines, phosphatidylcholines and sphingomyelins. <p>Conclusion A single measurement per individual may be sufficient for the study of 73% and 52% of the metabolites showing ICCs >0.50 in fasting and non-fasting samples, respectively. ICCs were higher in fasting samples that are preferable to non-fasting.en_US
dc.identifier.citationPLoS ONE 2015, 10:e0135437(8)en_US
dc.identifier.cristinIDFRIDAID 1332051
dc.identifier.doi10.1371/journal.pone.0135437
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/10037/8631
dc.identifier.urnURN:NBN:no-uit_munin_8254
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rights.accessRightsopenAccess
dc.subjectVDP::Medisinske Fag: 700en_US
dc.subjectVDP::Medical disciplines: 700en_US
dc.titleReliability of serum metabolites over a two-year period: A targeted metabolomic approach in fasting and non-fasting samples from EPICen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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