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dc.contributor.authorStriberny, Anja
dc.contributor.authorRavuri, Chandra Sekhar
dc.contributor.authorJobling, Malcolm
dc.contributor.authorJørgensen, Even Hjalmar
dc.date.accessioned2016-03-03T12:41:13Z
dc.date.available2016-03-03T12:41:13Z
dc.date.issued2015-09-30
dc.description.abstractThe highly seasonal anadromous Arctic charr (Salvelinus alpinus) was used to investigate the possible involvement of altered gene expression of brain neuropeptides in seasonal appetite regulation. Pro-opiomelanocortin (POMCA1, POMCA2), Cocaine and amphetamine regulated transcript (CART), Agouti related Peptide (AgRP), Neuropeptide Y (NPY) and Melanocortin Receptor 4 (MC4-R) genes were examined. The function of centrally expressed Leptin (Lep) in fish remains unclear, so Lep (LepA1, LepA2) and Leptin Receptor (LepR) genes were included in the investigation. In a ten months study gene expression was analysed in hypothalamus, mesencephalon and telencephalon of immature charr held under natural photoperiod (69°38’N) and ambient temperature and given excess feed. From April to the beginning of June the charr did not feed and lost weight, during July and August they were feeding and had a marked increase in weight and condition factor, and from November until the end of the study the charr lost appetite and decreased in weight and condition factor. Brain compartments were sampled from non-feeding charr (May), feeding charr (July), and non-feeding charr (January). Reverse transcription real-time quantitative PCR revealed temporal patterns of gene expression that differed across brain compartments. The non-feeding charr (May, January) had a lower expression of the anorexigenic LepA1, MC4-R and LepR in hypothalamus and a higher expression of the orexigenic NPY and AgRP in mesencephalon, than the feeding charr (July). In the telencephalon, LepR was more highly expressed in January and May than in July. These results do not indicate that changes in central gene expression of the neuropeptides investigated here directly induce seasonal changes in feeding in Arctic charr.en_US
dc.identifier.citationPLoS ONE 2015:1-13en_US
dc.identifier.cristinIDFRIDAID 1277504
dc.identifier.doi10.1371/journal.pone.0138857
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/10037/8646
dc.identifier.urnURN:NBN:no-uit_munin_8241
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138857
dc.rights.accessRightsopenAccess
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497en_US
dc.titleSeasonal Differences in Relative Gene Expression of Putative Central Appetite Regulators in Arctic Charr (Salvelinus alpinus) Do Not Reflect Its Annual Feeding Cycleen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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