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dc.contributor.authorMönkemöller, Viola
dc.contributor.authorØie, Cristina Ionica
dc.contributor.authorHubner, Wolfgang
dc.contributor.authorHuser, Thomas Rolf
dc.contributor.authorMcCourt, Peter Anthony
dc.date.accessioned2016-03-08T11:59:09Z
dc.date.available2016-03-08T11:59:09Z
dc.date.issued2015
dc.description.abstractLiver sinusoidal endothelial cells (LSECs) act as a filter between blood and the hepatocytes. LSECs are highly fenestrated cells; they contain transcellular pores with diameters between 50 to 200 nm. The small sizes of the fenestrae have so far prohibited any functional analysis with standard and advanced light microscopy techniques. Only the advent of super-resolution optical fluorescence microscopy now permits the recording of such small cellular structures. Here, we demonstrate the complementary use of two different super-resolution optical microscopy modalities, 3D structured illumination microscopy (3D-SIM) and single molecule localization microscopy in a common optical platform to obtain new insights into the association between the cytoskeleton and the plasma membrane that supports the formation of fenestrations. We applied 3D-SIM to multi-color stained LSECs to acquire highly resolved overviews of large sample areas. We then further increased the spatial resolution for imaging fenestrations by single molecule localization microscopy applied to select small locations of interest in the same sample on the same microscope setup. We optimized the use of fluorescent membrane stains for these imaging conditions. The combination of these techniques offers a unique opportunity to significantly improve studies of subcellular ultrastructures such as LSEC fenestrations.en_US
dc.descriptionPublished version. Source at <a href=http://doi.org/10.1038/srep16279>http://doi.org/10.1038/srep16279</a>.en_US
dc.identifier.citationScientific Reports 2015, 5en_US
dc.identifier.cristinIDFRIDAID 1287442
dc.identifier.doi10.1038/srep16279
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10037/8747
dc.identifier.urnURN:NBN:no-uit_munin_8348
dc.language.isoengen_US
dc.publisherNature Publishing Groupen_US
dc.rights.accessRightsopenAccess
dc.subjectActinen_US
dc.subjectApplied opticsen_US
dc.subjectMolecular biophysicsen_US
dc.subjectSuper-resolution microscopyen_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Biofysikk: 477en_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Biophysics: 477en_US
dc.titleMultimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrationsen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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