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dc.contributor.authorThode, Sunniva Katharina
dc.contributor.authorKahlke, Tim
dc.contributor.authorRobertsen, Espen Mikal
dc.contributor.authorHansen, Hilde
dc.contributor.authorHaugen, Peik
dc.date.accessioned2016-03-10T11:13:50Z
dc.date.available2016-03-10T11:13:50Z
dc.date.issued2015-02-04
dc.description.abstract<i>Background</i>: Iron is an essential micronutrient for all living organisms, and virulence and sequestration of iron in pathogenic bacteria are believed to be correlated. As a defence mechanism, potential hosts therefore keep the level of free iron inside the body to a minimum. In general, iron metabolism is well studied for some bacteria (mostly human or animal pathogens). However, this area is still under-investigated for a number of important bacterial pathogens. Aliivibrio salmonicida is a fish pathogen, and previous studies of this bacterium have shown that production of siderophores is temperature regulated and dependent on low iron conditions. In this work we studied the immediate changes in transcription in response to a sudden decrease in iron levels in cultures of A. salmonicida. In addition, we compared our results to studies performed with Vibrio cholerae and Vibrio vulnificus using a pan-genomic approach.<p> <p><i>Results</i>: Microarray technology was used to monitor global changes in transcriptional levels. Cultures of A. salmonicida were grown to mid log phase before the iron chelator 2,2’-dipyridyl was added and samples were collected after 15 minutes of growth. Using our statistical cut-off values, we retrieved thirty-two differentially expressed genes where the most up-regulated genes belong to an operon encoding proteins responsible for producing the siderophore bisucaberin. A subsequent pan-transcriptome analysis revealed that nine of the up-regulated genes from our dataset were also up-regulated in datasets from similar experiments using V. cholerae and V. vulnificus, thus indicating that these genes are involved in a shared strategy to mitigate low iron conditions.<p> <p><i>Conclusions</i>: The present work highlights the effect of iron limitation on the gene regulatory network of the fish pathogen A. salmonicida, and provides insights into common and unique strategies of Vibrionaceae species to mitigate low iron conditions.en_US
dc.descriptionSource at <a href=http://dx.doi.org/10.1186/s12866-015-0342-7>http://dx.doi.org/10.1186/s12866-015-0342-7</a> © Thode et al.; licensee BioMed Central. 2015en_US
dc.identifier.citationThode, S.K., Kahlke, T., Robertsen, E.M., Hansen, H. & Haugen, P. (2015). The immediate global responses of <i>Aliivibrio salmonicida</i> to iron limitations. <i>BMC Microbiology</i>, 15:9. https://doi.org/10.1186/s12866-015-0342-7en_US
dc.identifier.cristinIDFRIDAID 1292595
dc.identifier.doi10.1186/s12866-015-0342-7
dc.identifier.issn1471-2180
dc.identifier.urihttps://hdl.handle.net/10037/8843
dc.identifier.urnURN:NBN:no-uit_munin_8414
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.relation.ispartofThode, S.K. (2017). Iron dependent gene regulation and siderophore systems in <i>Vibrionaceae</i>. (Doctoral thesis). <a href=https://hdl.handle.net/10037/14984>https://hdl.handle.net/10037/14984. </a>
dc.rights.accessRightsopenAccess
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472en_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472en_US
dc.subjectAliivibrio salmonicidaen_US
dc.subjectIron homeostasisen_US
dc.subjectFerric uptake regulatoren_US
dc.subjectSiderophoreen_US
dc.subjectBisucaberinen_US
dc.subjectMicroarrayen_US
dc.titleThe immediate global responses of Aliivibrio salmonicida to iron limitationsen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US


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