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dc.contributor.authorLauksund, Reidunn Silje
dc.contributor.authorGreiner-Tollersrud, Linn
dc.contributor.authorChang, Chia Jung
dc.contributor.authorRobertsen, Børre
dc.date.accessioned2016-03-15T13:04:46Z
dc.date.available2016-03-15T13:04:46Z
dc.date.issued2015
dc.description.abstractInfectious pancreatic necrosis virus (IPNV) is one of the major viral pathogens causing disease in farmed Atlantic salmon worldwide. In the present work we show that several of the IPN proteins have powerful antagonistic properties against type I IFN induction in Atlantic salmon. Each of the five IPNV genes cloned into an expression vector were tested for the ability to influence activation of the Atlantic salmon IFNa1 promoter by the interferon promoter inducing protein one (IPS-1) or interferon regulatory factors (IRF). This showed that preVP2,VP3 andVP5 inhibited activation of both promoters, whileVP4 only antagonized activation of the IFNa1 promoter. The viral protease VP4 was the most potent inhibitor of IFN induction, apparently targeting the IRF1 and IRF3 branch of the signaling cascade. VP4 antagonism is independent of its protease activity since the catalytically dead mutant VP4K674A inhibited activation of the IFNa1 promoter to a similar extent as wild type VP4. In contrast to the other IPNV proteins, the RNA-dependent RNA polymerase VP1 activated the IFNa1 promoter. The ability to activate the IFN response was disrupted in the mutant VP1S163A, which has lost the ability to produce dsRNA. VP1 also exhibited synergistic effects with IRF1 and IRF3 in inducing an IFNa1-dependent antiviral state in cells. Taken together these results suggest that IPNV has developed multiple IFN antagonistic properties to prevent IFN-induction by VP1 and its dsRNA genome.en_US
dc.descriptionPublished version. Source at <a href=http://doi.org/10.1016/j.virusres.2014.11.018>http://doi.org/10.1016/j.virusres.2014.11.018</a>.en_US
dc.identifier.citationVirus Research 2015, 196:113-121en_US
dc.identifier.cristinIDFRIDAID 1224294
dc.identifier.doi10.1016/j.virusres.2014.11.018
dc.identifier.issn0168-1702
dc.identifier.urihttps://hdl.handle.net/10037/8966
dc.identifier.urnURN:NBN:no-uit_munin_8531
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rights.accessRightsopenAccess
dc.subjectIPNVen_US
dc.subjectInterferonen_US
dc.subjectAntagonismen_US
dc.subjectIPS-1en_US
dc.subjectIRF1en_US
dc.subjectIRF3en_US
dc.subjectVDP::Medisinske Fag: 700en_US
dc.titleInfectious pancreatic necrosis virus proteins VP2, VP3, VP4 and VP5 antagonize IFNa1 promoter activation while VP1 induces IFNa1en_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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