Blar i forfatter Artikler, rapporter og annet (kjemi) "Assefa, Netsanet Gizaw"
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Structural and biophysical analysis of interactions between cod and human uracil-DNA N-glycosylase (UNG) and UNG inhibitor (Ugi)
Assefa, Netsanet Gizaw; Niiranen, Laila; Johnson, Kenneth; Leiros, Hanna-Kirsti S.; Smalås, Arne O.; Willassen, Nils Peder; Moe, Elin (Journal article; Tidsskriftartikkel; Peer reviewed, 2014-07-25)Uracil-DNA N-glycosylase from Atlantic cod (cUNG) shows cold-adapted features such as high catalytic efficiency, a low temperature optimum for activity and reduced thermal stability compared with its mesophilic homologue human UNG (hUNG). In order to understand the role of the enzyme–substrate interaction related to the cold-adapted properties, the structure of cUNG in complex with a bacteriophage ... -
A unique class I polyhydroxyalkanoate synthase (PhaC) from Brevundimonas sp. KH11J01 exists as a functional trimer: A comparative study with PhaC from Cupriavidus necator H16
Assefa, Netsanet Gizaw; Hansen, Hilde; Altermark, Bjørn (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-05-06)Polyhydroxyalkanoates (PHAs) are natural biodegradable polyesters that are produced by numerous prokaryotic microorganisms primarily as a carbon- and energy reserve. The PhaC enzyme catalyzes the last step in the PHA biosynthesis pathway and synthesizes PHA polymers from hydroxyalkanoic acids. A type I PhaC from a PHAproducing marine bacterium Brevundimonas sp. KH11J01 (BrPhaC) was identified, ...