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dc.contributor.authorBerg, Kristel
dc.contributor.authorPedersen, Hege Lynum
dc.contributor.authorLeiros, Ingar
dc.date.accessioned2021-03-09T08:18:56Z
dc.date.available2021-03-09T08:18:56Z
dc.date.issued2020-07-09
dc.description.abstractIron is an essential nutrient for bacteria, however its propensity to form toxic hydroxyl radicals at high intracellular concentrations, requires its acquisition to be tightly regulated. Ferric uptake regulator (Fur) is a metal-dependent DNA-binding protein that acts as a transcriptional regulator in maintaining iron metabolism in bacteria and is a highly interesting target in the design of new antibacterial drugs. Fur mutants have been shown to exhibit decreased virulence in infection models. The protein interacts specifically with DNA at binding sites designated as ‘Fur boxes’. In the present study, we have investigated the interaction between Fur from the fish pathogen <i>Aliivibrio salmonicida</i> (AsFur) and its target DNA using a combination of biochemical and in silico methods. A series of target DNA oligomers were designed based on analyses of Fur boxes from other species, and affinities assessed using electrophoretic mobility shift assay. Binding strengths were interpreted in the context of homology models of AsFur to gain molecular-level insight into binding specificity.en_US
dc.identifier.citationBerg K, Pedersen hl, Leiros I. Biochemical characterization of ferric uptake regulator (Fur) from Aliivibrio salmonicida. Mapping the DNA sequence specificity through binding studies and structural modelling. Biometals. 2020en_US
dc.identifier.cristinIDFRIDAID 1823630
dc.identifier.doi10.1007/s10534-020-00240-6
dc.identifier.issn0966-0844
dc.identifier.issn1572-8773
dc.identifier.urihttps://hdl.handle.net/10037/20653
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.relation.journalBiometals
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Mathematics and natural science: 400en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400en_US
dc.titleBiochemical characterization of ferric uptake regulator (Fur) from Aliivibrio salmonicida. Mapping the DNA sequence specificity through binding studies and structural modellingen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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