dc.contributor.author | Christensen, Mikkel | |
dc.contributor.author | Jablonski, Piotr | |
dc.contributor.author | Altermark, Bjørn | |
dc.contributor.author | Irgum, Knut | |
dc.contributor.author | Hansen, Hilde | |
dc.date.accessioned | 2022-02-17T12:34:38Z | |
dc.date.available | 2022-02-17T12:34:38Z | |
dc.date.issued | 2021-12-20 | |
dc.description.abstract | Background: Several members of the bacterial Halomonadacea family are natural producers of polyhydroxyalkanoates (PHA), which are promising materials for use as biodegradable bioplastics. Type-strain species of Cobetia
are designated PHA positive, and recent studies have demonstrated relatively high PHA production for a few strains
within this genus. Industrially relevant PHA producers may therefore be present among uncharacterized or less
explored members. In this study, we characterized PHA production in two marine Cobetia strains. We further analyzed
their genomes to elucidate pha genes and metabolic pathways which may facilitate future optimization of PHA production in these strains.<p>
Results: Cobetia sp. MC34 and Cobetia marina DSM 4741<sup>T</sup>
were mesophilic, halotolerant, and produced PHA from
four pure substrates. Sodium acetate with- and without co-supplementation of sodium valerate resulted in high PHA
production titers, with production of up to 2.5 g poly(3-hydroxybutyrate) (PHB)/L and 2.1 g poly(3-hydroxybutyrateco-3-hydroxyvalerate) (PHBV)/L in Cobetia sp. MC34, while C. marina DSM 4741<sup>T</sup>
produced 2.4 g PHB/L and 3.7 g
PHBV/L. Cobetia marina DSM 4741<sup>T</sup>
also showed production of 2.5 g PHB/L from glycerol. The genome of Cobetia sp.
MC34 was sequenced and phylogenetic analyses revealed closest relationship to Cobetia amphilecti. PHA biosynthesis
genes were located at separate loci similar to the arrangement in other Halomonadacea. Further genome analyses
revealed some diferences in acetate- and propanoate metabolism genes between the two strains. Interestingly, only
a single PHA polymerase gene (phaC<sub>2</sub>) was found in Cobetia sp. MC34, in contrast to two copies (phaC<sub>1</sub> and phaC<sub>2</sub>) in
C. marina DSM 4741<sup>T</sup>
. In silico analyses based on phaC genes show that the PhaC<sub>2</sub> variant is conserved in Cobetia and
contains an extended C-terminus with a high isoelectric point and putative DNA-binding domains.<p>
Conclusions: Cobetia sp. MC34 and C. marina DSM 4741<sup>T</sup>
are natural producers of PHB and PHBV from industrially relevant pure substrates including acetate. However, further scale up, optimization of growth conditions, or use of metabolic engineering is required to obtain industrially relevant PHA production titers. The putative role of the Cobetia
PhaC<sub>2</sub> variant in DNA-binding and the potential implications remains to be addressed by in vitro- or in vivo methods. | en_US |
dc.identifier.citation | Christensen, Jablonski, Altermark, Irgum, Hansen. High natural PHA production from acetate in Cobetia sp. MC34 and Cobetia marina DSM 4741<sup>T</sup> and in silico analyses of the genus specific PhaC<inf>2</inf> polymerase variant. Microbial Cell Factories. 2021;20(1):1-23 | en_US |
dc.identifier.cristinID | FRIDAID 1975163 | |
dc.identifier.doi | 10.1186/s12934-021-01713-0 | |
dc.identifier.issn | 1475-2859 | |
dc.identifier.uri | https://hdl.handle.net/10037/24080 | |
dc.language.iso | eng | en_US |
dc.publisher | BMC | en_US |
dc.relation.journal | Microbial Cell Factories | |
dc.rights.accessRights | openAccess | en_US |
dc.rights.holder | Copyright 2021 The Author(s) | en_US |
dc.title | High natural PHA production from acetate in Cobetia sp. MC34 and Cobetia marina DSM 4741T and in silico analyses of the genus specific PhaC2 polymerase variant | en_US |
dc.type.version | publishedVersion | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |
dc.type | Peer reviewed | en_US |