| dc.description.abstract | We have studied the innate immune responses of Atlantic salmon and how this responds to
stimulation and infection by the aquatic orthomyxovirus Infectious salmon anemia virus
(ISAV). This work has focused on the Type I interferon (IFN) system which is critically
important in the vertebrate immune response against viral infections. As a part of this work we have studied the Atlantic salmon IFN regulatory factor 7 (IRF7). In mammals IRF7 is the major regulator of IFN transcription in response to viral infections, and our results show that the salmon homolog performs similar tasks. Furthermore, we have investigated the stimulatory effect of two synthetic ligands that induce the IFN system by mimicking viral infections in the cell and compared this to the immune response against ISAV. Our results indicate that both specialized immune cells and non-immune cells induce the IFN system in response to these treatments, but we also show that ISAV is able to interfere with the host` IFN response in order to successfully replicate. Finally, our studies show that the ISAV
genome encodes two genes, S7ORF1 and S8ORF2, which is involved in antagonizing the IFN
response during infections. | en |
| dc.description | The papers of the thesis are not available in Munin:
<br>1. Kileng, Ø., Brundtland, M. I. and Robertsen, B.: «Infectious salmon anemia virus is a powerful inducer of key genes of the type I interferon system of Atlantic salmon, but is not inhibited by interferon», Fish & Shellfish immunology 23(2007); 378-389 (Elsevier - publisher's restriction). Available at <a href=http://dx.doi.org/10.1016/j.fsi.2006.11.011>http://dx.doi.org/10.1016/j.fsi.2006.11.011</a>
<br>2. Kileng, Ø., Albuquerque, A. and Robertsen, B.: «Induction of interferon system genes in Atlantic salmon by the imidazoquinoline S-27609, a ligand for Toll-like receptor 7», Fish & Shellfish immunology 24(2007); 514-522 (Elsevier - publisher's restriction). Available at <a href=http://dx.doi.org/10.1016/j.fsi.2007.10.005>http://dx.doi.org/10.1016/j.fsi.2007.10.005</a>
<br>3. García-Rosado, E., Markussen, T., Kileng, Ø., Baekkevold, E. S., Robertsen, B., Mjaaland, S. and Rimstad, E.: «Molecular and functional characterization of two infectious salmon anaemia virus (ISAV) proteins with type I interferon antagonizing activity», Virus Research 133(2008); 228-238 (Elsevier - publisher's restriction). Available at <a href=http://dx.doi.org/10.1016/j.virusres.2008.01.008>http://dx.doi.org/10.1016/j.virusres.2008.01.008</a>
<br>4. Kileng, Ø., Workenhe, S. T., Bergan, V. and Robertsen, B.: «An Atlantic salmon IRF-7 gene identified by analysis of sequence and function and its expression compared with a STAT1 gene». Manuscript, later published in Developmental and Comparative Immunology 33(2009); 18-27 (Elsevier), published version available at <a href=http://dx.doi.org/10.1016/j.dci.2008.07.020>http://dx.doi.org/10.1016/j.dci.2008.07.020</a> | en |
English
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