Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens
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https://hdl.handle.net/10037/25101Date
2022-01-27Type
Journal articleTidsskriftartikkel
Peer reviewed
Abstract
Vibrio natriegens has recently gained attention as a novel fast-growing bacterium in
synthetic biology applications. Currently, a limited set of genetic elements optimised for
Escherichia coli are used in V. natriegens due to the lack of DNA parts characterised in this
novel host. In this study, we report the identification and cross-characterisation of artificial
promoters and 5′ untranslated regions (artificial regulatory sequence, ARES) that lead to
production of fluorescent proteins with a wide-range of expression levels. We identify and
cross-characterise 52 constructs in V. natriegens and E. coli. Furthermore, we report the
DNA sequence and motif analysis of the ARESs using various algorithms. With this study,
we expand the pool of characterised genetic DNA parts that can be used for different
biotechnological applications using V. natriegens as a host microorganism.
Publisher
Frontiers MediaCitation
Tietze L, Hoff MW, Lale R. Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens. Frontiers in Bioengineering and Biotechnology. 2022Metadata
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