Insights into the role of three Endonuclease III enzymes for oxidative stress resistance in the extremely radiation resistant bacterium Deinococcus radiodurans
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https://hdl.handle.net/10037/31789Date
2023-09-12Type
Journal articleTidsskriftartikkel
Peer reviewed
Author
Rollo, Filipe; Martins, Guilherme D.; Gouveia, André G.; Ithurbide, Solenne; Servant, Pascale; Romão, Célia V.; Moe, ElinAbstract
The extremely radiation and desiccation resistant bacterium Deinococcus
radiodurans possesses three genes encoding Endonuclease III-like enzymes
(DrEndoIII1, DrEndoIII2, DrEndoIII3). In vitro enzymatic activity measurements
revealed that DrEndoIII2 is the main Endonuclease III in this organism, while
DrEndoIII1 and 3 possess unusual and, so far, no detectable EndoIII activity,
respectively. In order to understand the role of these enzymes at a cellular level,
DrEndoIII knockout mutants were constructed and subjected to various oxidative
stress related conditions. The results showed that the mutants are as resistant to
ionizing and UV-C radiation as well as H2O2 exposure as the wild type. However,
upon exposure to oxidative stress induced by methyl viologen, the knockout
strains were more resistant than the wild type. The difference in resistance
may be attributed to the observed upregulation of the EndoIII homologs gene
expression upon addition of methyl viologen. In conclusion, our data suggest that
all three EndoIII homologs are crucial for cell survival in stress conditions, since
the knockout of one of the genes tend to be compensated for by overexpression
of the genes encoding the other two.
Publisher
Frontiers MediaCitation
Rollo, Martins, Gouveia, Ithurbide, Servant, Romão, Moe. Insights into the role of three Endonuclease III enzymes for oxidative stress resistance in the extremely radiation resistant bacterium Deinococcus radiodurans. Frontiers in Microbiology. 2023;14Metadata
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