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Properties and distribution of a metallo-β-lactamase (ALI-1) from the fish pathogen Aliivibrio salmonicida LFI1238

Permanent lenke
https://hdl.handle.net/10037/9090
DOI
https://doi.org/10.1093/jac/dku433
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Åpne
article.pdf (337.0Kb)
accepted manuscript version (PDF)
Dato
2015
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Forfatter
Kristiansen, Anders; Grgic, Miriam; Altermark, Bjørn; Leiros, Ingar
Sammendrag
Objectives. To characterize the chromosome-encoded metallo-β-lactamase (MBL) from the psychrophilic, marine fish-pathogenic bacterium Aliivibrio salmonicida LFI1238 and check for the presence of the gene in other Aliivibrio isolates both connected to the fish-farming industry and from the environment. Methods. The MBL gene was cloned and intracellularly expressed in Escherichia coli. Kinetic parameters, NaCl dependence, pH optimum and temperature optimum were determined using purified enzyme. The VIM-2 enzyme from a Pseudomonas aeruginosa hospital isolate was used as a counterpart in comparative analysis. PCRs with degenerate MBL primers were used to screen different A. salmonicida isolates for the presence of the gene. Results. A. salmonicida MBL (ALI-1) is an Ambler class B β-lactamase sharing 39% and 29% amino acid identity with IMP-1 and VIM-2, respectively. ALI-1 hydrolysed all β-lactam antibiotics tested, except for the monobactam aztreonam and the penicillin piperacillin. A profound increase in activity was observed when adding NaCl to the assay mixture (60% active without addition of NaCl, increasing to 100% at 0.5 M NaCl). The increase was less noticeable for VIM-2 (100% active at 0.2 M NaCl). ALI-1 appears to be ubiquitous in nature as it is found in Aliivibrio isolates not affected by human activity. Conclusions. This work provides more data for the ever-expanding MBL group of enzymes. These periplasmic enzymes are activated by addition of NaCl, and the marine enzyme is highly salt tolerant and cold active. The observed enzyme properties very likely reflect the conditions that the enzymes face in situ.
Beskrivelse
Accepted manuscript version. Published version at http://doi.org/10.1093/jac/dku433.
Forlag
Oxford University Press
Sitering
Journal of Antimicrobial Chemotherapy 2015, 70(3):766-772
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