Blar i forfatter "McCourt, Peter Anthony"

Viser treff 21-28 av 28

    • Opportunities and Challenges for Nanotherapeutics for the Aging Population 

      Hunt, Nicholas J; McCourt, Peter Anthony; Kuncic, Zdenka; Le Couteur, David G; Cogger, Victoria C (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-01-25)
      Nanotherapeutics utilize the properties of nanomaterials to alter the pharmacology of the drugs and therapies being transported, leading to changes in their biological disposition (absorption, distribution, cellular uptake, metabolism and elimination) and ultimately, their pharmacological effect. This provides an opportunity to optimize the pharmacology of drugs, particularly for those that are ...

    • Quantitative analysis methods for studying fenestrations in liver sinusoidal endothelial cells. A comparative study 

      Szafranska, Karolina; Holte, Christopher Florian; Kruse, Larissa Dorothea; Mao, Hong; Øie, Cristina Ionica; Symonski, Marek; Zapotoczny, Bartlomiej; McCourt, Peter Anthony (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-28)
      Liver Sinusoidal Endothelial Cells (LSEC) line the hepatic vasculature providing blood filtration via transmembrane nanopores called fenestrations. These structures are 50−300 nm in diameter, which is below the resolution limit of a conventional light microscopy. To date, there is no standardized method of fenestration image analysis. With this study, we provide and compare three different approaches: ...

    • Quantum Dot Nanomedicine Formulations Dramatically Improve Pharmacological Properties and Alter Uptake Pathways of Metformin and Nicotinamide Mononucleotide in Aging Mice 

      Hunt, Nick; Lockwood, Glen P; Kang, Sun Woo; Westwood, Lara; Limantoro, Christina; Chrzanowski, Wojciech; McCourt, Peter Anthony; Kuncic, Zdenka; Le Couteur, David G; Cogger, V.C. (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-02-24)
      Orally administered Ag<sub>2</sub>S quantum dots (QDs) rapidly cross the small intestine and are taken up by the liver. Metformin and nicotinamide mononucleotide (NMN) target metabolic and aging processes within the liver. This study examined the pharmacology and toxicology of QD-based nanomedicines as carriers of metformin and NMN in young and old mice, determining if their therapeutic potency and ...

    • Rapid Intestinal Uptake and Targeted Delivery to the Liver Endothelium Using Orally Administered Silver Sulfide Quantum Dots. 

      Hunt, Nicholas J; Lockwood, Glen P; Le Couteur, Frank H.; McCourt, Peter Anthony; Singla, Nidhi; Kang, Sun; Burgess, Andrew; Kuncic, Zdenka; Le Couteur, David G; Cogger, Victoria C (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-01-24)
      Quantum dots (QDs) are used for imaging and transport of therapeutics. Here we demonstrate rapid absorption across the small intestine and targeted delivery of QDs with bound materials to the liver sinusoidal endothelial cells (LSECs) or hepatocytes in vitro and in vivo following oral administration. QDs were radiolabeled with <sup>3</sup> H-oleic acid, with a fluorescent tag or <sup>14</su ...

    • The scavenger function of liver sinusoidal endothelial cells in health and disease 

      Bhandari, Sabin; Larsen, Anett Kristin; McCourt, Peter Anthony; Smedsrød, Bård; Sørensen, Karen Kristine (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-10-11)
      The aim of this review is to give an outline of the blood clearance function of the liver sinusoidal endothelial cells (LSECs) in health and disease. Lining the hundreds of millions of hepatic sinusoids in the human liver the LSECs are perfectly located to survey the constituents of the blood. These cells are equipped with high-affinity receptors and an intracellular vesicle transport apparatus, ...

    • Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages 

      Bhandari, Sabin; Li, Ruomei; Simon-Santamaria, Jaione; McCourt, Peter Anthony; Johansen, Steinar Daae; Smedsrød, Bård; Martinez, Inigo Zubiavrre; Sørensen, Karen Kristine (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-11-27)
      <i>Background</i> - Liver sinusoidal endothelial cells (LSECs) and Kupffer cells (KCs; liver resident macrophages) form the body’s most effective scavenger cell system for the removal of harmful blood-borne substances, ranging from modified self-proteins to pathogens and xenobiotics. Controversies in the literature regarding the LSEC phenotype pose a challenge when determining distinct functionalities ...

    • Tuning of Liver Sieve: The Interplay between Actin and Myosin Regulatory Light Chain Regulates Fenestration Size and Number in Murine Liver Sinusoidal Endothelial Cells 

      Zapotoczny, Barlomiej; Szafranska, Karolina; Lekka, Malgorzata; Ahluwalia, Balpreet Singh; McCourt, Peter Anthony (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-08-30)
      Liver sinusoidal endothelial cells (LSECs) facilitate the efficient transport of macromolecules and solutes between the blood and hepatocytes. The efficiency of this transport is realized via transcellular nanopores, called fenestrations. The mean fenestration size is 140 ± 20 nm, with the range from 50 nm to 350 nm being mostly below the limits of diffraction of visible light. The cellular ...

    • Uptake and Degradation of Bacteriophages by Liver Sinusoidal Endothelial Cells 

      Wolfson, Deanna; Øie, Cristina Ionica; Yasunori, Tanji; Dumitriu, Gianina; McCourt, Peter Anthony; Sørensen, Karen Kristine; Smedsrød, Bård; Ahluwalia, Balpreet Singh (Conference object; Konferansebidrag, 2018)
      <p>Bacteriophages (briefly, “phages”) are viruses which target bacteria, and are non-infectious to eukaryotic cells. It is estimated that more than 30 billion phages cross into the human body from the gut each day1, and eventually need to be cleared from the blood circulation. The liver plays a central role in pathogen clearance, and liver sinusoidal endothelial cells (LSECs), which form the lining ...