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Quantitative analysis methods for studying fenestrations in liver sinusoidal endothelial cells. A comparative study

Permanent link
https://hdl.handle.net/10037/24137
DOI
https://doi.org/10.1016/j.micron.2021.103121
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Date
2021-07-28
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Author
Szafranska, Karolina; Holte, Christopher Florian; Kruse, Larissa Dorothea; Mao, Hong; Øie, Cristina Ionica; Symonski, Marek; Zapotoczny, Bartlomiej; McCourt, Peter Anthony
Abstract
Liver Sinusoidal Endothelial Cells (LSEC) line the hepatic vasculature providing blood filtration via transmembrane nanopores called fenestrations. These structures are 50−300 nm in diameter, which is below the resolution limit of a conventional light microscopy. To date, there is no standardized method of fenestration image analysis. With this study, we provide and compare three different approaches: manual measurements, a semi-automatic (threshold-based) method, and an automatic method based on user-friendly open source machine learning software. Images were obtained using three super resolution techniques – atomic force microscopy (AFM), scanning electron microscopy (SEM), and structured illumination microscopy (SIM). Parameters describing fenestrations such as diameter, area, roundness, frequency, and porosity were measured. Finally, we studied the user bias by comparison of the data obtained by five different users applying provided analysis methods.
Is part of
Szafranska, K. (2022). Novel screening methods for nanoscale changes in liver cell fenestrations elicited by pharmaceuticals. (Doctoral thesis). https://hdl.handle.net/10037/25981.
 
Holte, C.F. (2024). Novel insights into the fenestrated scavenger endothelium of the liver sinusoid. (Doctoral thesis). https://hdl.handle.net/10037/33068
Publisher
Elsevier
Citation
Szafranska K, Holte C, Kruse LD, Mao H, Øie CI, Symonski, Zapotoczny B, McCourt PAG. Quantitative analysis methods for studying fenestrations in liver sinusoidal endothelial cells. A comparative study. Micron. 2021;150:103121
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