Recent additions

  • Grading of oral squamous cell carcinomas – Intra and interrater agreeability: Simpler is better? 

    Steigen, Sonja E; Søland, Tine Merete; Nginamau, Elisabeth Sivy; Laurvik, Helene; Costea, Daniela-Elena; Johannessen, Anne Christine; Jebsen, Peter Wilhelm; Bjerkli, Inger-Heidi; Uhlin-Hansen, Lars; Hadler-Olsen, Elin Synnøve (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-01-03)
    <i>Background</i> - Numerous studies have been presented on histological grading of oral squamous cell carcinomas (OSCC) for predicting survival, but uncertainty of their usefulness rises due to discordances of results. A scoring system should be robust and well validated, and intra‐ and interrater agreement can be used as a tool to visualize the strength of reproducibility.<p><p> <i>Methods</i> ...
  • Responsiveness to PD-1 Blockade in End-Stage Colon Cancer with Gene Locus 9p24.1 Copy-Number Gain 

    Ree, Anne Hansen; Nygaard, Vigdis; Russnes, Hege Elisabeth Giercksky; Heinrich, Daniel; Nygaard, Vegard; Johansen, Christin; Bergheim, Inger Riise; Hovig, Eivind; Beiske, Klaus; Negård, Anne; Børresen-Dale, Anne-Lise; Flatmark, Kjersti; Mælandsmo, Gunhild Mari (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-02-25)
    Most patients whose large bowel cancer has spread to other organs do not respond to immune therapy. We detected a rare gene mutation, termed 9p24.1 copy-number gain (CNG), in an otherwise incurable colorectal cancer that provoked an immune therapy response. We identified this gene mutation by gene-panel sequencing of DNA from a liver metastasis biopsy from a patient who had disease refractory to ...
  • Human concentrations of uric acid scavenges adaptive and maladaptive ROS in isolated rat hearts subjected to ischemic stress 

    Boardman, Neoma Tove; Falck, Aleksander Tank; Lund, Trine; Chu, X; Martin, Armas Maria Montserrat; Norvik, Jon Viljar; Jenssen, Trond Geir; Ytrehus, Kirsti (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-09-14)
    Uric acid is a purine degradation product but also an important antioxidant and ROS scavenger. Experimental settings that mimic myocardial ischemia-reperfusion have not included uric acid despite that it is always present in human extracellular fluid and plasma. We hypothesized that uric acid has an important role in myocardial ROS scavenging. Here, we tested the cardiac response to uric acid on ...
  • Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography 

    Hadler-Olsen, Elin Synnøve; Winberg, Jan-Olof (Journal article; Tidsskriftartikkel; Peer reviewed, 2019)
    To explore the physiological or pathological roles of proteases, it is important to be able to detect and precisely localize them in a tissue, to differentiate between inactive and active forms, as well as to quantify and determine the nature of the enzyme that degrades a given substrate. Here we present an in situ gelatin zymography method that allows for a precise localization of active gelatin-degrading ...
  • Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography 

    Hadler-Olsen, Elin Synnøve; Winberg, Jan-Olof (Journal article; Tidsskriftartikkel; Peer reviewed, 2019)
    To explore the physiological or pathological roles of proteases, it is important to be able to detect and precisely localize them in a tissue, to differentiate between inactive and active forms, as well as to quantify and determine the nature of the enzyme that degrades a given substrate. Here we present a protocol for real-time gelatin zymography that is very useful for the detection of gelatin-degrading ...
  • Regulation of gap junction intercellular communication by connexin ubiquitination: physiological and pathophysiological implications 

    Totland, Max; Rasmussen, Nikoline Lander; Knudsen, Lars Mørland; Leithe, Edward (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-09-09)
    Gap junctions consist of arrays of intercellular channels that enable adjacent cells to communicate both electrically and metabolically. Gap junctions have a wide diversity of physiological functions, playing critical roles in both excitable and non-excitable tissues. Gap junction channels are formed by integral membrane proteins called connexins. Inherited or acquired alterations in connexins are ...
  • Exposures and Health Effects of Bioaerosols in Seafood Processing Workers - a Position Statement 

    Bonlokke, Jakob; Bang, Berit; Aasmoe, Lisbeth; Rahman, Anas M. Abdel; Syron, Laura N.; Andersson, Eva; Dahlman-Høglund, Anna; Lopata, Andreas L.; Jeebhay, Mohamed Fareed (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-08-27)
    Occupational hazards exist in the processing of seafood both in land-based facilities as well as on board vessels. Recent findings on occupational injury and respiratory health risks among seafood processing workers were presented and discussed at the IFISH5 conference. Particular emphasis was put on the challenges that im/migrant workers encounter, the greater risks onboard factory vessels, especially ...
  • Optimisation of high-resolution fluorescence microscopy of normal- and preeclamptic placentas 

    Knutsen, Kristine Marie Bakken (Master thesis; Mastergradsoppgave, 2019-08-14)
    Preeclampsia (PE) affects 3-5 % of pregnant women and may lead to maternal and/or fetal death. The main theory of PE is placental ischemia, leading to a dysfunctional placenta and clinical signs as hypertension and proteinuria in the mother. The primary aim of the thesis was to implement and optimise a method for high-resolution microscopy of placental cryo-sections. Secondary aims were to compare ...
  • A Novel Truncated Form of Nephronectin Is Present in Small Extracellular Vesicles Isolated from 66cl4 Cells 

    Toraskar, Jimita Prashant; Norvoll Magnussen, Synnøve; Hagen, Lars; Sharma, Animesh; Hoang, Linh; Bjørkøy, Geir; Svineng, Gunbjørg; Steigedal, Tonje S. (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-02-01)
    Extracellular vesicles are emerging as biomarkers in breast cancer. Our recent report suggested that an intracellular granular staining pattern of the extracellular matrix protein nephronectin (NPNT) in breast tumor sections correlated with a poor prognosis. Furthermore, the results showed that NPNT is localized in extracellular vesicles derived from mouse breast cancer cells. In this study, we ...
  • Biological stain collection – Absorbing paper is superior to cotton swabs 

    Janssen, Kirstin; Aune, Marthe; Olsen, Marita; Olsen, Gunn-Hege; Berg, Thomas (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-10-14)
    Biological evidence at crime scenes often contains very small amounts of DNA. Therefore, it is important to use the most effective sampling devices and procedures for stain collection. Currently, cotton swabs moistened with water are widely used, also in our laboratory. However, several studies have shown that other methods may be more efficient.<p> <p>In this study, we compared the DNA sampling ...
  • MiRNAs‐181a/b therapy in lung cancer: reality or myth? 

    Braicu, Cornelia; Gulei, Diana; Cojocneanu, Roxana Maria; Raduly, Lajos; Jurj, Ancuta; Knutsen, Erik; Calin, George Adrian; Berindan-Neagoe, Ioana (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-01-03)
    Despite substantial progress in oncology, lung cancer remains the number one malignancy in terms of both incidence and mortality rates, and there thus remains an urgent need for new therapeutic alternatives. MicroRNA (miRNA) have an important role in cancer initiation and progression due to their capacity to interfere with transcriptional signaling and regulate key cellular processes. miR‐181a and ...
  • Genetic relationships of European, Mediterranean, and SW Asian populations using a panel of 55 AISNPs 

    Pakstis, Andrew J.; Gurkan, Cemal; Dogan, Mustafa; Balkaya, Hasan Emin; Dogan, Serkan; Neophytou, Pavlos I.; Cherni, Lotfi; Boussetta, Sami; Khodjet-El-Khil, Houssein; ElGaaied, Amel Ben Ammar; Salvo, Nina Mjølsnes; Janssen, Kirstin; Olsen, Gunn-Hege; Hadi, Sibte; Almohammed, Eida Khalaf; Pereira, Vania; Truelsen, Ditte Mikkelsen; Bulbul, Ozlem; Soundararajan, Usha; Rajeevan, Haseena; Kidd, Judith R.; Kidd, Kenneth K. (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-07-08)
    The set of 55 ancestry informative SNPs (AISNPs) originally developed by the Kidd Lab has been studied on a large number of populations and continues to be applied to new population samples. The existing reference database of population samples allows the relationships of new population samples to be inferred on a global level. Analyses show that these autosomal markers constitute one of the better ...
  • Significance of progesterone receptors (PR-A and PR-B) expression as predictors for relapse after successful therapy of endometrial hyperplasia: a retrospective cohort study 

    Sletten, Elise Thoresen; Arnes, Marit; Lyså, Lena Marianne Myreng; Larsen, Marthe; Ørbo, Anne (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-01-25)
    <p><i>Objective - </i>After successful progestin therapy for endometrial hyperplasia (EH), the risk of relapse remains. We aimed to assess if immunohistochemical (IHC) expression of progesterone receptor isoforms, PR‐A and PR‐B, in endometrial glands and stroma in pre‐treatment endometrial biopsies was related to relapse of EH. <p><i>Design and setting - </i>Biopsy material originated from women ...
  • Design of galardine analogs as putative psudolysin inhibitors based on ab initio fragment molecular orbital calculations 

    Ezawa, Takuya; Sugiyama, Satoshi; Ara, Ayami; Sylte, Ingebrigt; Kurita, Noriyuki (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-08-29)
    Pseudolysin (PLN) is a metalloproteinase secreted from bacteria that degrades extracellular proteins to produce bacterial nutrition. It is thus expected that inhibitors against PLN can suppress the growth of bacteria and their pandemic spread. In addition, since these inhibitors do not attack to bacteria directly, there is a reduced risk for producing drug-resistant bacteria. On the other hand, as ...
  • Disentangling the immune response and host-pathogen interactions in Francisella noatunensis infected Atlantic cod 

    Solbakken, Monica Hongrø; Jentoft, Sissel; Reitan, Trond; Mikkelsen, Helene; Gregers, Tone Fredsvik; Bakke, Oddmund; Jakobsen, Kjetill Sigurd; Seppola, Marit (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-04-13)
    The genetic repertoire underlying teleost immunity has been shown to be highly variable. A rare example is Atlantic cod and its relatives Gadiformes that lacks a hallmark of vertebrate immunity: Major Histocompatibility Complex class II. No immunological studies so far have fully unraveled the functionality of this particular immune system. Through global transcriptomic profiling, we investigate the ...
  • Whole transcriptome analysis of the Atlantic cod vaccine response reveals subtle changes in adaptive immunity 

    Solbakken, Monica Hongrø; Jentoft, Sissel; Reitan, Trond; Mikkelsen, Helene; Jakobsen, Kjetill Sigurd; Seppola, Marit (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-05-28)
    Atlantic cod has lost the Major Histocompatibility complex class II pathway – central to pathogen presentation, humoral response and immunity. Here, we investigate the immunological response of Atlantic cod subsequent to dip vaccination with <i>Vibrio anguillarum</i> bacterin using transcriptome sequencing. The experiment was conducted on siblings from an Atlantic cod family found to be highly ...
  • Molecular determinants regulating selective binding of autophagy adapters and receptors to ATG8 proteins 

    Wirth, Martina; Zhang, Wenxin; Razi, Minoo; Nyoni, Lynet; Joshi, Dhira; O'Reilly, Nicola; Johansen, Terje; Tooze, Sharon; Mouilleron, Stephane (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-05-03)
    Autophagy is an essential recycling and quality control pathway. Mammalian ATG8 proteins drive autophagosome formation and selective removal of protein aggregates and organelles by recruiting autophagy receptors and adaptors that contain a LC3-interacting region (LIR) motif. LIR motifs can be highly selective for ATG8 subfamily proteins (LC3s/GABARAPs), however the molecular determinants regulating ...
  • MMP9 modulates the metastatic cascade and immune landscape for breast cancer anti-metastatic therapy 

    Owyong, Mark; Chou, Jonathan; van den Bijgaart, Renske; Kong, Niwen; Efe, Gizem; Maynard, Carrie; Talmi-Frank, Dalit; Solomonov, Inna; Koopman, Charlotte; Hadler-Olsen, Elin Synnøve; Headley, Mark; Lin, Charlene; Wang, Chih-Yang; Sagi, Irit; Werb, Zena; Plaks, Vicki (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-11-14)
    Metastasis, the main cause of cancer-related death, has traditionally been viewed as a late-occurring process during cancer progression. Using the MMTV-PyMT luminal B breast cancer model, we demonstrate that the lung metastatic niche is established early during tumorigenesis. We found that matrix metalloproteinase 9 (MMP9) is an important component of the metastatic niche early in tumorigenesis and ...
  • Predicting human plasma concentrations of persistent organic pollutants from dietary intake and socio-demographic information in the Norwegian Women and Cancer study 

    Berg, Vivian; Nøst, Therese Haugdahl; Sandanger, Torkjel M; Rylander, Charlotta (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-11-09)
    <i>Background</i> - Concentrations of persistent organic pollutants (POPs) in humans are influenced by a large number of factors including birth year, reproductive history and diet. Accordingly, information on dietary habits and socio-demographic variables may predict plasma concentrations of POPs, thus enabling studies on health effects in large epidemiological studies, without performing time ...
  • Circulating tumor cells as a tool for assessing tumor heterogeneity 

    Tellez Gabriel, Marta; Heymann, Marie-Françoise; Heymann, Dominique (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-06-19)
    Tumor heterogeneity is the major cause of failure in cancer prognosis and prediction. Accurately detecting heterogeneity for the development of biomarkers and the detection of the clones resistant to therapy is one of the main goals of contemporary medicine. Metastases belong to the natural history of cancer. The present review gives an overview on the origin of tumor heterogeneity. Recent progress ...

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