Recent additions

  • Biogeographical Ancestry Analyses Using the ForenSeq™ DNA Signature Prep Kit and Multiple Prediction Tools 

    Salvo, Nina Mjølsnes; Olsen, Gunn-Hege; Berg, Thomas; Janssen, Kirstin (Journal article; Tidsskriftartikkel, 2024-04-18)
    The inference of biogeographical ancestry (BGA) can assist in police investigations of serious crime cases and help to identify missing people and victims of mass disasters. In this study, we evaluated the typing performance of 56 ancestry-informative SNPs in 177 samples using the ForenSeq™ DNA Signature Prep Kit on the MiSeq FGx system. Furthermore, we compared the prediction accuracy of the tools ...
  • Long term 2D-culture of primary mouse liver sinusoidal endothelial cells (LSEC) 

    Abujayyab, Basema B.A. (Master thesis; Mastergradsoppgave, 2023-05-31)
    Liver sinusoidal endothelial cells (LSEC) are unique and specialized cell type with high endocytic activity of bloodborne waste macromolecules. LSEC lack basal lamina and present a unique structural feature called fenestrations, which facilitate passive filtering and exchange of nutrients with other liver cells. Developing LSEC cell lines is challenging with regards to preserving their morphology ...
  • Co-culturing with Streptococcus anginosus alters Staphylococcus aureus transcriptome when exposed to tonsillar cells 

    Bastakoti, Srijana; Pesonen, Maiju; Ajayi, Clément; Julin, Kjersti; Corander, Jukka; Johannessen, Mona Susan; Hanssen, Anne-Merethe Christiansen (Journal article; Tidsskriftartikkel, 2024-01-25)
    <p><i>Introduction:</i> Improved understanding of <i>Staphylococcus aureus</i> throat colonization in the presence of other co-existing microbes is important for mapping <i>S. aureus</i> adaptation to the human throat, and recurrence of infection. Here, we explore the responses triggered by the encounter between two common throat bacteria, <i>S. aureus</i> and <i>Streptococcus anginosus</i>, to ...
  • Periodontitis is associated with decreased experimental pressure pain tolerance: The Tromsø Study 2015–2016 

    Hadler-Olsen, Elin Synnøve; Petrenya, Natalia; Jönsson, Birgitta; Steingrímsdóttir, Ólöf Anna; Stubhaug, Audun; Nielsen, Christopher Sivert (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-03-01)
    Aim - To assess the relationship between periodontitis and experimental pain tolerance.<p> <p>Materials and Methods - Participants from the population-based seventh survey of the Tromsø Study with data on periodontitis were included (n = 3666, 40–84 years old, 51.6% women). Pain tolerance was assessed through (i) pressure pain tolerance (PPT) test with a computerized cuff pressure algometry on ...
  • Moving sperm forward 

    Hellsten, Benjamin (Mastergradsoppgave; Master thesis, 2023-05-26)
    Objective: The purpose of this study was to investigate a manual sperm sorting method (Swim-up) and a non-invasive microfluidics method (Zymot microfluidics) with emphasis on formation of reactive oxygen species (ROS) and DNA fragmentation as objective measurements of spermatozoa quality. Standard phase contrast imaging of spermatozoa was compared with quantitative phase contrast microscopy (QPM) ...
  • Characterization of extracellular vesicles from U-2OS15E, a human osteosarcoma cell line persistently infected by BK polyomavirus 

    Logendran, Aumkar (Mastergradsoppgave; Master thesis, 2021-05-25)
    The human polyomavirus BKPyV infects most people worldwide and establishes a lifelong persistent infection in the renourinary tract. The virus rarely gives symptoms in healthy people, but it can cause severe disease under certain immunocompromised states. Despite its high prevalence, our knowledge about how the virus evades host immunity and persists still poor. Extracellular vesicles (EVs) ...
  • 4-nitroquinoline 1-oxide-induced oral epithelial lesions exhibit time- and stage-dependent changes in the tumor immune microenvironment 

    Sellæg, Kjersti; Schwienbacher, Ruth; Kranz, Mathias; Engan Aamodt, Anna; Wirsing, Anna Maria; Berge, gerd; Hadler-Olsen, Elin Synnøve; Norvoll Magnussen, Synnøve (Journal article; Tidsskriftartikkel; Peer reviewed, 2024-05-15)
    Oral tongue squamous cell carcinoma (OTSCC) is the most common cancer of the oral cavity and is associated with high morbidity due to local invasion and lymph node metastasis. Tumor infiltrating lymphocytes (TILs) are associated with good prognosis in oral cancer patients and dictate response to treatment. Ectopic sites for immune activation in tumors, known as tertiary lymphoid structures ...
  • Duration and dynamics of Klebsiella pneumoniae species complex gut carriage in a community-based adult population cohort 

    Kabir, Wasifa (Master thesis; Mastergradsoppgave, 2023-05-15)
    Klebsiella pneumoniae species complex (KpSC) is a group of closely related bacterial species including Klebsiella pneumoniae sensu stricto known as a major human opportunistic pathogen associated with healthcare. KpSC gut carriage is a major risk factor for clinical infections and elderly people (≥60y) are at the risk of KpSC gut colonisation. This master project investigated the presence, duration ...
  • Characterization of competition between commensal A2 and clinical A1 strains of Enterococcus faecium 

    Fenzel, Carolin Kornelia (Mastergradsoppgave; Master thesis, 2023-05-14)
    Enterococcus faecium (E. faecium) emerged from a gut commensal to one of the leading nosocomial multidrug-resistant pathogens. The species of E. faecium, has a deep phylogenetic split into commensal and clinical strains. The dominance of clinical strains during nosocomial infections is only insufficiently understood. Previous studies revealed competitive characteristics between the commensal ...
  • Exploring the adenosine system by cancer-associated fibroblasts in the context of radiotherapy 

    Afroj, Shamima (Mastergradsoppgave; Master thesis, 2023-05-14)
    Background of the study: Cancer-associated fibroblasts (CAFs) are a heterogeneous population of cells that are mainly found in solid malignancies and play vital roles in tumor progression and metastasis, including important immunoregulatory functions. In this study, our objective was to explore the immunosuppressive adenosine system within the CAFs isolated from non-small cell lung carcinomas (NSCLC) ...
  • The inflammation repressor TNIP1/ABIN-1 is degraded by autophagy following TBK1 phosphorylation of its LIR 

    Rasmussen, Nikoline Lander; Zhou, Jianwen; Olsvik, Hallvard Lauritz; Kaeser-Pebernard, Stéphanie; Lamark, Trond; Dengjel, Joern; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-03-09)
    The inflammatory repressor TNIP1/ABIN-1 is important for keeping in check inflammatory and cell-death pathways to avoid potentially dangerous sustained activation of these pathways. We have now found that TNIP1 is rapidly degraded by selective macroautophagy/autophagy early (0–4 h) after activation of TLR3 by poly(I:C)-treatment to allow expression of pro-inflammatory genes and proteins. A few hours ...
  • Atg8 family proteins, LIR/AIM motifs and other interaction modes 

    Rogov, Vladimir V.; Nezis, Ioannis P.; Tsapras, P; Zhang, Hong; Dagdas, Yasin; Noda, Nobuo N; Nakatogawa, Hitoshi; Wirth, Martina; Mouilleron, Stephane; McEwan, David G.; Behrends, Christian; Deretic, Vojo P.; Elazar, Zvulun; Tooze, Sharon A.; Dikic, Ivan; Lamark, Trond; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-03-19)
    The Atg8 family of ubiquitin-like proteins play pivotal roles in autophagy and other processes involving vesicle fusion and transport where the lysosome/vacuole is the end station. Nuclear roles of Atg8 proteins are also emerging. Here, we review the structural and functional features of Atg8 family proteins and their protein-protein interaction modes in model organisms such as yeast, Arabidopsis, ...
  • LC3B is a cofactor for LMX1B-mediated transcription of autophagy genes in dopaminergic neurons 

    Kournoutis, Athanasios; Johansen, Terje (Journal article; Tidsskriftartikkel, 2023-04-10)
    It is becoming increasingly clear that the Atg8 family of autophagy proteins have roles not only in the cytoplasm, but also in the cell nucleus. In this issue, Jimenez-Moreno et al. (2023. J. Cell Biol. <a href=https://doi.org/10.1083/jcb.201910133>https://doi.org/10.1083/jcb.201910133</a>) report that nuclear LC3B binds to the LIM homeodomain transcription factor LMX1B and acts as a cofactor for ...
  • Dritbra» hvis ungdom gir oss avføringen sin 

    Pettersen, Veronika Kucharová; Kloos, Julia Maria (Others; Andre, 2024-02-17)
    En forskningsgruppe ved UiT samler inn avføring fra barn og unge for å studere tarmbakterier. Målet er å utvikle bedre diagnoser for sykdommer og oppdage lidelser tidligere, ifølge medisinstudent Ole Martin Ellingsbø Nilsen. Prosjektet har fått økonomisk støtte fra Barnekreftforeningen og rekrutteringshjelp fra Tromsø kommune. Ungdommer som deltar får en «kit» for å samle inn prøvene, samt ...
  • The first tigecycline resistant Enterococcus faecium in Norway was related to tigecycline exposure 

    Hegstad, Kristin; Pöntinen, Anna Kaarina; Bjørnholt, Jørgen; Paulsen, Else Quist; Sundsfjord, Arnfinn Ståle (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-12-19)
    Objectives - We describe the first tigecycline resistant enterococcal isolate in Norway and the mechanisms involved.<p> <p>Material and methods - The Norwegian National Advisory Unit on Detection of Antimicrobial Resistance (K-res). received in 2022 an Enterococcus faecium blood culture isolate with decreased susceptibility to tigecycline from a hospitalized patient in the South-Eastern Norway ...
  • Homology Modeling of Transporter Proteins 

    Sylte, Ingebrigt; Gabrielsen, Mari; Kristiansen, kurt (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-03-24)
    Membrane transporter proteins are divided into channels/pores and carriers and constitute protein families of physiological and pharmacological importance. Several presently used therapeutic compounds elucidate their effects by targeting membrane transporter proteins, including anti-arrhythmic, anesthetic, antidepressant, anxiolytic and diuretic drugs. The lack of three-dimensional structures of ...
  • Climate Change Causes Molecular-Level Stress Responses 

    Urbarova, Ilona (Journal article; Tidsskriftartikkel, 2019)
    Anemones are abundant in an acidified ocean. But are they thriving? New genomics research shows that ocean acidification puts these animals under considerable stress. The good news is that some marine animals can tolerate the impacts of increased carbon dioxide levels in seawater.
  • Biochemical and Cellular Characterization of the Function of Fluorophosphonate-Binding Hydrolase H (FphH) in Staphylococcus aureus Support a Role in Bacterial Stress Response 

    Fellner, Matthias; Walsh, Annabel; Ahator, Stephen Dela; Aftab, Nadia; Sutherland, Ben; Tan, Eng W; Bakker, Alexander T; Martin, Nathaniel I; van der Stelt, Mario; Lentz, Christian Stephan (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-10-12)
    The development of new treatment options for bacterial infections requires access to new targets for antibiotics and antivirulence strategies. Chemoproteomic approaches are powerful tools for profiling and identifying novel druggable target candidates, but their functions often remain uncharacterized. Previously, we used activity-based protein profiling in the opportunistic pathogen Staphylococcus ...
  • Emergence of vancomycin-resistant enterococci from vancomycin susceptible enterococci in hospitalized patients under antimicrobial therapy 

    Janice, Jessin; Wagner, Theresa Maria; Olsen, Karina; Hegstad, Joachim; Hegstad, Kristin (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-12-19)
    Objectives: Enterococci are opportunistic pathogens with plastic genomes that evolve, acquire, and transmit antimicrobial-resistant determinants such as vancomycin resistance clusters. While vancomycinresistant enterococci (VRE) have emerged as successful nosocomial pathogens, the mechanism by which vancomycin-susceptible enterococci (VSE) transform to VRE in hospitalized patients remains ...
  • The first vanE-type vancomycin resistant Enterococcus faecalis isolates in Norway – phenotypic and molecular characteristics 

    al Rubaye, Mushtaq Talib Shawi; Janice, Jessin; Bjørnholt, Jørgen; Löhr, Iren Høyland; Sundsfjord, Arnfinn Ståle; Hegstad, Kristin (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-12-27)
    Objectives - We aimed to characterize the vanE cluster and its genetic support in the first Norwegian vanE-type isolates and assess genetic relatedness to other vanE isolates.<p> <p>Methods - Two vanE-type vancomycin resistant Enterococcus faecalis (vanE-VREfs) isolates (E1 and E2) recovered from the same patient 30 months apart were examined for antimicrobial susceptibility, genome sequence, ...

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