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norskInstitutt for medisinsk biologi
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Evaluation of the One Health-Ness of 20 Years of Antimicrobial Resistance Surveillance in Norway
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-06-21)We evaluated the One Health-ness (OH-ness) of the surveillance system for antimicrobial resistance (AMR) in Norway by using the recently developed “Evaluation tool for One Health epidemiological surveillance capacities and capabilities” (OH–EpiCap tool). First, we defined the Norwegian AMR surveillance system that we would evaluate. The tool was applied by a group of stakeholders (key persons in the ... -
SLE classification criteria: Is “The causality principle” integrated and operative – and do the molecular and genetical network, on which criteria depend on, support the definition of SLE as “a one disease entity” – A theoretical discussion
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-10-24)Molecular and cellular aspects of the autoimmune pathophysiology in SLE is linked to the “The causality principle”. SLE Classification Criteria identify per definition disease measures (here: synonymous with classification criteria), but not diagnostic criteria within a classical framework. These two mostly theoretical criteria collections represent a salient conflict between phenomenology and the ... -
Activity-Based Protein Profiling in Methicillin-Resistant Staphylococcus aureus Reveals the Broad Reactivity of a Carmofur-Derived Probe
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-08-08)Activity-based protein profiling is a powerful chemoproteomic technique to detect active enzymes and identify targets and off-targets of drugs. Here, we report the use of carmofur- and activity-based probes to identify biologically relevant enzymes in the bacterial pathogen Staphylococcus aureus. Carmofur is an anti-neoplastic prodrug of 5-fluorouracil and also has antimicrobial and anti-biofilm ... -
Infection of liver sinusoidal endothelial cells with Muromegalovirus muridbeta1 involves binding to neuropilin-1 and is dynamin-dependent
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-11-09)Liver sinusoidal endothelial cells (LSEC) are scavenger cells with a remarkably high capacity for clearance of several blood-borne macromolecules and nanoparticles, including some viruses. Endocytosis in LSEC is mainly via the clathrin-coated pit mediated route, which is dynamin-dependent. LSEC can also be a site of infection and latency of betaherpesvirus, but mode of virus entry into these ... -
Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIB, yet maintain a preserved fenestration the Glmp gt/gt mouse model of slowly progressing liver fibrosis
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-11-01)Liver sinusoidal endothelial cells (LSECs) are fenestrated endothelial cells with a unique, high endocytic clearance capacity for blood-borne waste macromolecules and colloids. This LSEC scavenger function has been insufficiently characterized in liver disease. The Glmp<sup>gt/gt</sup> mouse lacks expression of a subunit of the MFSD1/GLMP lysosomal membrane protein transporter complex, is born ... -
High-speed TIRF and 2D super-resolution structured illumination microscopy with a large field of view based on fiber optic components
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-08-16)Super-resolved structured illumination microscopy (SR-SIM) is among the most flexible, fast, and least perturbing fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-built instruments are easily able to deliver two-fold resolution enhancement at video-rate frame rates, but the cost of the instruments is still relatively high, and the physical size ... -
Anti–miR-93-5p therapy prolongs sepsis survival by restoring the peripheral immune response
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-06-01)Sepsis remains a leading cause of death for humans and currently has no pathogenesis-specific therapy. Hampered progress is partly due to a lack of insight into deep mechanistic processes. In the past decade, deciphering the functions of small noncoding miRNAs in sepsis pathogenesis became a dynamic research topic. To screen for new miRNA targets for sepsis therapeutics, we used samples for miRNA ... -
Årlig driftsrapport 2017. Norsk overvåkingssystem for antibiotikaresistens hos mikrober (NORM)
(Research report; Forskningsrapport, 2018)NORM har i 2017 ferdigutviklet og publisert en egen interaktiv kartløsning, NORM-atlas, for presentasjon av NORM-databasen online. I det web-baserte NORM-atlaset kan man lage en tilpasset og dynamisk rapport for forekomst av antibiotikaresistens ved å velge geografisk område og tidsperiode i tillegg til type klinisk isolat, mikrobe og antimikrobielt middel. Denne nye applikasjonen gjør det raskt og ... -
Kvalitetssikring av genteknologiske metoder
(Research report; Forskningsrapport, 2018-09)Kvalitetskontroll av infeksjonsserologiske metoder var tema for strategimøtet i 2016 og det er dermed naturlig å følge opp med kvalitetssikring av molekylærgenetiske metoder i år. På møtet i fjor ble det gitt detaljerte anbefalinger om validering/verifisering, internkontroller, usikkerhetsbedømmelse, gråsoner og tolkning. Genteknologiske metoder skiller seg betydelig fra serologi, og kvalitetssikr ... -
Driftsrapport 2018 - Norsk overvåkingssystem for antibiotikaresistens hos mikrober NORM
(Research report; Forskningsrapport, 2019)Driftsrapporten er en standardisert rapport som gir grunnlag for en samlet oversikt over driften av helseregistrene Folkehelseinstituttet er dataansvarlig og databehandler for. Driftsrapporten brukes også blant annet til å oppfylle enkelte av helseregistrenes forskriftsfestede meldeplikt til Datatilsynet. -
Antibiotikaresistens - Kunnskapshull, utfordringer og aktuelle tiltak. Status 2020.
(Research report; Forskningsrapport, 2021)Antimikrobiell resistens (AMR) er en global trussel mot helse og velferd hos mennesker og dyr. Den grunnleggende forståelsen av AMR har ikke endret seg over det siste tiåret, men vi har fått mer detaljert innsikt i hvordan resistens oppstår, selekteres og kan spre seg innad hos og mellom mennesker, dyr og i miljøet. Det er nødvendig å se utfordringene knyttet til AMR i et helhetlig Én helse - ... -
Kvalitetskontroll av serologiske analyser. Virologisk-serologisk strategimøte 1. november 2001
(Research report; Forskningsrapport, 2001) -
Nationwide, population-based observational study of the molecular epidemiology and temporal trend of carbapenemase-producing Enterobacterales in Norway, 2015 to 2021
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-07-06)National and regional carbapenemaseproducing Enterobacterales (CPE) surveillance is essential to understand the burden of antimicrobial resistance, elucidate outbreaks, and develop infection-control or antimicrobial-treatment recommendations. Aim: This study aimed to describe CPE and their epidemiology in Norway from 2015 to 2021. Methods: A nationwide, population-based observational study of ... -
CALCOCO1 is a soluble reticulophagy receptor
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-07-25)The endoplasmic reticulum (ER) is the largest membrane-bound organelle in eukaryotic cells and plays critical roles in diverse processes in metabolism, signaling and intracellular organization. In response to stress stimuli such as nutrient deprivation, accumulation of misfolded proteins or exposure to chemicals, the ER increases in size through upregulated synthesis of its components to counteract ... -
Immunization with lytic polysaccharide monooxygenase CbpD induces protective immunity against Pseudomonas aeruginosa pneumonia
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-07-17)Pseudomonas aeruginosa (PA) CbpD belongs to the lytic polysaccharide monooxygenases (LPMOs), a family of enzymes that cleave chitin or related polysaccharides. Here, we demonstrate a virulence role of CbpD in PA pneumonia linked to impairment of host complement function and opsonophagocytic clearance. Following intratracheal challenge, a PA ΔCbpD mutant was more easily cleared and produced less ... -
Selective autophagy mediated by autophagic adapter proteins
(Journal article; Tidsskriftartikkel; Peer reviewed, 2011-03-01)Mounting evidence suggests that autophagy is a more selective process than originally anticipated. The discovery and characterization of autophagic adapters, like p62 and NBR1, has provided mechanistic insight into this process. p62 and NBR1 are both selectively degraded by autophagy and able to act as cargo receptors for degradation of ubiquitinated subtstrates. A direct interaction between these ... -
The long noncoding RNA CCAT2 induces chromosomal instability through BOP1-AURKB signaling
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-08-15)Background & Aims - Chromosomal instability (CIN) is a carcinogenesis event that promotes metastasis and resistance to therapy by unclear mechanisms. Expression of the colon cancer–associated transcript 2 gene (CCAT2), which encodes a long noncoding RNA (lncRNA), associates with CIN, but little is known about how CCAT2 lncRNA regulates this cancer enabling characteristic.<p> <p>Methods - We ... -
Protonation states of central amino acids in a zinc metalloprotease complexed with inhibitor: Molecular mechanics optimizations and ab initio molecular orbital calculations
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-04-01)The zinc-metalloprotease pseudolysin (PLN) secreted from bacteria degrades extracellular proteins to produce bacterial nutrition. Since PLN has a Zn ion at the inhibitor-binding site, the interactions between Zn and PLN residues as well as inhibitor can be significantly changed depending on the protonation states of PLN residues at the inhibitor-binding site. To determine stable protonation states ... -
Liver X receptors induce antiproliferative effects in basal-like breast cancer
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-06-21)Liver X receptors (LXRs) are nuclear transcription factors important in the regulation of cholesterol transport, and glucose and fatty acid metabolism. The antiproliferative role of LXRs has been studied in a variety of malignancies and may represent a therapeutic opportunity in cancers lacking targeted therapies, such as triple-negative breast cancer. In this study, we investigated the impact of ... -
Intraregional hospital outbreak of OXA-244-producing Escherichia coli ST38 in Norway, 2020
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-07-06)Infections with OXA-244-carbapenemase-producing Escherichia coli with sequence type (ST)38 have recently increased in Europe. Due to its low-level activity against carbapenems, OXA-244 can be difficult to detect. Previous assessments have not revealed a clear source and route of transmission for OXA-244-producing E. coli, but there are indications of non-healthcare related sources and community ...
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