Blar i forfatter "Opstad, Ida Sundvor"

Viser treff 21-23 av 23

    • Three-dimensional structured illumination microscopy data of mitochondria and lysosomes in cardiomyoblasts under normal and galactose-adapted conditions 

      Opstad, Ida Sundvor; Godtliebsen, Gustav; Ströhl, Florian; Myrmel, Truls; Ahluwalia, Balpreet Singh; Agarwal, Krishna; Birgisdottir, Åsa birna (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-03-23)
      This three-dimensional structured illumination microscopy (3DSIM) dataset was generated to highlight the suitability of 3DSIM to investigate mitochondria-derived vesicles (MDVs) in H9c2 cardiomyoblasts in living or fxed cells. MDVs act as a mitochondria quality control mechanism. The cells were stably expressing the tandem-tag eGFP-mCherry-OMP25-TM (outer mitochondrial membrane) which can be used ...

    • Virtual labeling of mitochondria in living cells using correlative imaging and physics-guided deep learning 

      Somani, Ayush; Sekh, Arif Ahmed; Opstad, Ida Sundvor; Birgisdottir, Åsa birna; Myrmel, Truls; Ahluwalia, Balpreet Singh; Horsch, Alexander; Agarwal, Krishna; Prasad, Dilip K. (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-09-28)
      Mitochondria play a crucial role in cellular metabolism. This paper presents a novel method to visualize mitochondria in living cells without the use of fluorescent markers. We propose a physics-guided deep learning approach for obtaining virtually labeled micrographs of mitochondria from bright-field images. We integrate a microscope’s point spread function in the learning of an adversarial neural ...

    • A waveguide imaging platform for live-cell TIRF imaging of neurons over large fields of view 

      Opstad, Ida Sundvor; Ströhl, Florian; Fantham, Marcus J.; Hockings, Colin; Vanderpoorten, Oliver; Tartwijk, Francesca W. van; Qiaojin Lin, Julie; Tinguely, Jean-Claude; Dullo, Firehun Tsige; Kaminski‐Schierle, Gabriele S.; Ahluwalia, Balpreet Singh; Kaminski, Clemens F. (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-02-17)
      Large fields of view (FOVs) in total internal reflection fluorescence microscopy (TIRFM) via waveguides have been shown to be highly beneficial for single molecule localisation microscopy on fixed cells [1,2] and have also been demonstrated for short‐term live‐imaging of robust cell types [3‐5], but not yet for delicate primary neurons nor over extended periods of time. Here, we present a waveguide‐based ...