A waveguide imaging platform for live-cell TIRF imaging of neurons over large fields of view
AuthorOpstad, Ida Sundvor; Ströhl, Florian; Fantham, Marcus J.; Hockings, Colin; Vanderpoorten, Oliver; Tartwijk, Francesca W. van; Qiaojin Lin, Julie; Tinguely, Jean-Claude; Dullo, Firehun Tsige; Kaminski‐Schierle, Gabriele S.; Ahluwalia, Balpreet Singh; Kaminski, Clemens F.
Large fields of view (FOVs) in total internal reflection fluorescence microscopy (TIRFM) via waveguides have been shown to be highly beneficial for single molecule localisation microscopy on fixed cells [1,2] and have also been demonstrated for short‐term live‐imaging of robust cell types [3‐5], but not yet for delicate primary neurons nor over extended periods of time. Here, we present a waveguide‐based TIRFM set‐up for live‐cell imaging of demanding samples. Using the developed microscope, referred to as the ChipScope, we demonstrate successful culturing and imaging of fibroblasts, primary rat hippocampal neurons and axons of Xenopus retinal ganglion cells (RGCs). The high contrast and gentle illumination mode provided by TIRFM coupled with the exceptionally large excitation areas and superior illumination homogeneity offered by photonic waveguides have potential for a wide application span in neuroscience applications.
CitationOpstad IS, Ströhl F, Fantham MJ, Hockings, Vanderpoorten O, Tartwijk, Qiaojin Lin, Tinguely J, Dullo FT, Kaminski‐Schierle, Ahluwalia BS, Kaminski CF. A waveguide imaging platform for live-cell TIRF imaging of neurons over large fields of view. Journal of Biophotonics. 2020
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