Show simple item record

dc.contributor.advisorMartinez, Inigo Zubiavrre
dc.contributor.advisorBerzaghi, Rodrigo
dc.contributor.advisorStuge, Tor Brynjar
dc.contributor.authorTornaas, Stian
dc.date.accessioned2019-06-25T08:09:03Z
dc.date.available2019-06-25T08:09:03Z
dc.date.issued2019-05-15
dc.description.abstractStromal cells in the tumor microenvironment (TME) interacts with cancer cells and affect their behavior in diverse manners. In this study, we investigated in vitro the mechanisms by which non-irradiated and irradiated cancer-associated fibroblasts (CAFs) isolated from patients with non-small cell lung carcinoma (NSCLC) affected the phenotype and function of monocyte-derived dendritic cells (Mo-DCs) isolated from blood samples gathered for healthy blood donors. The expression of CD14, CD1a and CD209 was lower in Mo-DCs in co-culture with CAFs than in control cells. Further, the expression of co-stimulatory molecules CD80 and CD86 was down-regulated in culture with CAF supernatant. For functional markers CD40 and HLA-DR, we could observe that expression was suppressed in CAF-CM and co-culture experiments. Our results demonstrated that CAF-secreted molecules could suppress antigen uptake capacity and migratory ability for Mo-DCs. For Mo-DCs priming of CD4+ T-cell, we could see that both CAF-CM and co-culture conditions blocked the priming of T-cells. Irradiation of CAFs with fractioned medium-high dose induced in some settings enhanced expression of surface markers as well as increased functionality of Mo-DCs. This study demonstrates that there is a cross-talk between CAFs and Mo-DCs were results indicates that CAFs educate Mo-DCs to become tolerogenic.en_US
dc.identifier.urihttps://hdl.handle.net/10037/15599
dc.language.isoengen_US
dc.publisherUiT Norges arktiske universiteten_US
dc.publisherUiT The Arctic University of Norwayen_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Author(s)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)en_US
dc.subject.courseIDMBI-3911
dc.subjectVDP::Medisinske Fag: 700::Helsefag: 800en_US
dc.subjectVDP::Medical disciplines: 700::Health sciences: 800en_US
dc.titleModulation of dendritic cells phenotype and functions by irradiated and non-irradiated lung cancer fibroblasts. An in vitro study.en_US
dc.typeMaster thesisen_US
dc.typeMastergradsoppgaveen_US


File(s) in this item

Thumbnail
Thumbnail

This item appears in the following collection(s)

Show simple item record

Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)