Isolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent site
Permanent link
https://hdl.handle.net/10037/10810Date
2016-02-24Type
Journal articleTidsskriftartikkel
Peer reviewed
Author
Wissuwa, Juliane; Stokke, Runar; Fedøy, Anita-Elin; Lian, Kjersti; Smalås, Arne O.; Steen, Ida HeleneAbstract
Members of the genus Geobacillus have been isolated from a wide variety of habitats worldwide and are the
subject for targeted enzyme utilization in various industrial applications. Here we report the isolation and
complete genome sequence of the thermophilic starch-degrading Geobacillus sp. 12AMOR1. The strain 12AMOR1 was
isolated from deep-sea hot sediment at the Jan Mayen hydrothermal Vent Site. Geobacillus sp. 12AMOR1 consists of a
3,410,035 bp circular chromosome and a 32,689 bp plasmid with a G + C content of 52 % and 47 %, respectively. The
genome comprises 3323 protein-coding genes, 88 tRNA species and 10 rRNA operons. The isolate grows on a
suite of sugars, complex polysaccharides and proteinous carbon sources. Accordingly, a versatility of genes
encoding carbohydrate-active enzymes (CAZy) and peptidases were identified in the genome. Expression,
purification and characterization of an enzyme of the glycoside hydrolase family 13 revealed a starch-degrading
capacity and high thermal stability with a melting temperature of 76.4 °C. Altogether, the data obtained point to a new
isolate from a marine hydrothermal vent with a large bioprospecting potential.
Description
Published version. Source at http://doi.org/10.1186/s40793-016-0137-y. License CC BY 4.0.