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Chip-based wide field-of-view nanoscopy

Permanent link
https://hdl.handle.net/10037/11998
DOI
https://doi.org/10.1038/nphoton.2017.55
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Accepted manuscript version (PDF)
Date
2017-04-24
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Author
Ahluwalia, Balpreet Singh; Helle, Øystein Ivar; Diekmann, Robin; Øie, Cristina Ionica; McCourt, Peter A. G.; Schuttpelz, Mark
Abstract
Present optical nanoscopy techniques use a complex microscope for imaging and a simple glass slide to hold the sample. Here, we demonstrate the inverse: the use of a complex, but mass-producible optical chip, which hosts the sample and provides a waveguide for the illumination source, and a standard low-cost microscope to acquire super-resolved images via two different approaches. Waveguides composed of a material with high refractive-index contrast provide a strong evanescent field that is used for single-molecule switching and fluorescence excitation, thus enabling chip-based single-molecule localization microscopy. Additionally, multimode interference patterns induce spatial fluorescence intensity variations that enable fluctuation-based super-resolution imaging. As chip-based nanoscopy separates the illumination and detection light paths, total-internal-reflection fluorescence excitation is possible over a large field of view, with up to 0.5 mm × 0.5 mm being demonstrated. Using multicolour chip-based nanoscopy, we visualize fenestrations in liver sinusoidal endothelial cells.
Description
Accepted manuscript version. Published version available at http://doi.org/10.1038/nphoton.2017.55.
Is part of
Helle, Ø.I. (2019). On-chip optical nanoscopy: towards high throughput and multi-modality. (Doctoral thesis). https://hdl.handle.net/10037/16641.
Publisher
Nature Publishing Group
Citation
Diekmann, R., Helle, Ø.I., Øie, C.I., McCourt, P., Huser, T.R, Schüttpelz, M. & Ahluwalia, B.S. (2017). Chip-based wide field-of-view nanoscopy. Nature Photonics, 11, 322-328. http://doi.org/10.1038/nphoton.2017.55
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