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dc.contributor.authorOlsen, Stian
dc.contributor.authorKrause, Kirsten
dc.date.accessioned2018-02-14T08:11:42Z
dc.date.available2018-02-14T08:11:42Z
dc.date.issued2017-04-27
dc.description.abstractThe parasitic vines of the genus Cuscuta form haustoria that grow into other plants and connect with their vascular system, thus allowing the parasite to feed on its host. A major obstacle that meets the infection organ as it penetrates the host tissue is the rigid plant cell wall. In the present study, we examined the activity of xyloglucan endotransglucosylases/hydrolases (XTHs) during the host-invasive growth of the haustorium. The level of xyloglucan endotransglucosylation (XET) activity was found to peak at the penetrating stage of Cuscuta reflexa on its host Pelargonium zonale. In vivo colocalization of XET activity and donor substrate demonstrated XET activity at the border between host and parasite. A test for secretion of XET-active enzymes from haustoria of C. reflexa corroborated this and further indicated that the xyloglucan-modifying enzymes originated from the parasite. A known inhibitor of XET, Coomassie Brilliant Blue R250, was shown to reduce the level of XET in penetrating haustoria of C. reflexa. Moreover, the coating of P. zonale petioles with the inhibitor compound lowered the number of successful haustorial invasions of this otherwise compatible host plant. The presented data indicate that the activity of Cuscuta XTHs at the host-parasite interface is essential to penetration of host plant tissue.en_US
dc.descriptionSource at <a href=https://doi.org/10.1371/journal.pone.0176754> https://doi.org/10.1371/journal.pone.0176754 </a>.en_US
dc.identifier.citationOlsen, S. & Krause, K. (2017). Activity of xyloglucan endotransglucosylases/hydrolases suggests a role during host invasion by the parasitic plant Cuscuta reflexa. PLoS ONE. 12(4).en_US
dc.identifier.issn1932-6203
dc.identifier.otherFRIDAID 1473092
dc.identifier.other10.1371/journal.pone.0176754
dc.identifier.urihttps://hdl.handle.net/10037/12154
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.journalPLoS ONE
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Parasittologi: 484en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480::Parasitology: 484en_US
dc.titleActivity of xyloglucan endotransglucosylases/hydrolases suggests a role during host invasion by the parasitic plant Cuscuta reflexaen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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