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dc.contributor.authorDubey, Vishesh
dc.contributor.authorAhmad, Azeem
dc.contributor.authorSingh, Rajwinder
dc.contributor.authorWolfson, Deanna
dc.contributor.authorBasnet, Purusotam
dc.contributor.authorAcharya, Ganesh
dc.contributor.authorMehta, Dalip Singh
dc.contributor.authorAhluwalia, Balpreet Singh
dc.date.accessioned2019-03-28T11:37:15Z
dc.date.available2019-03-28T11:37:15Z
dc.date.issued2018-07-24
dc.description.abstractTotal internal reflection fluorescence (TIRF) microscopy benefits from high-sensitivity, low background noise, low photo-toxicity and high-contrast imaging of sub-cellular structures close to the membrane surface. Although, TIRF microscopy provides high-contrast imaging it does not provide quantitative information about morphological features of the biological cells. Here, we propose an integrated waveguide chip-based TIRF microscopy and label-free quantitative phase imaging (QPI). The evanescent field present on top of a waveguide surface is used to excite the fluorescence and an upright microscope is used to collect the signal. The upright microscope is converted into a Linnik-type interferometer to sequentially extract both the quantitative phase information and TIRF images of the cells. Waveguide chip-based TIRF microscopy benefits from decoupling of illumination and collection light path, large field of view imaging and pre-aligned configuration for multi-color TIRF imaging. The proposed multi-modal microscopy is used to study inflammation caused by lipopolysaccharide (LPS) on rat macrophages. The TIRF microscopy showed that LPS inflammatory molecule disrupts the cell membrane and causes cells to significantly expand across a substrate. While, QPI module quantified changes in the sub-cellular content of the LPS challenged macrophages, showing a net decrease in its maximum phase values.en_US
dc.description.sponsorshipNorwegian Centre for International Cooperation in Education (SIU) University Grant Commission (UGC)en_US
dc.descriptionSource at <a href=https://doi.org/10.1364/OE.26.019864>https://doi.org/10.1364/OE.26.019864. </a> © 2018 Optical Society of America under the terms of the <a href=https://www.osapublishing.org/library/license_v1.cfm#VOR-OA>OSA Open Access Publishing Agreement. </a>Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.en_US
dc.identifier.citationDubey, V., Ahmad, A., Singh, R., Wolfson, D.L., Basnet, P., Acharya, G., ... Ahluwalia, B.S. (2018). Multi-modal chip-based fluorescence and quantitative phase microscopy for studying inflammation in macrophages. <i>Optics Express, 26</i>(16), 19864-19876. https://doi.org/10.1364/OE.26.019864en_US
dc.identifier.cristinIDFRIDAID 1613560
dc.identifier.doi10.1364/OE.26.019864
dc.identifier.issn1094-4087
dc.identifier.urihttps://hdl.handle.net/10037/15089
dc.language.isoengen_US
dc.publisherOptical Society of Americaen_US
dc.relation.journalOptics Express
dc.relation.projectIDEC/FP7: European Research Council, (Project number 336716)en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/336716/EU/NANOSCOPY/en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectNear-field microscopyen_US
dc.subjectFluorescence microscopyen_US
dc.subjectMedical and biological imagingen_US
dc.subjectVDP::Mathematics and natural science: 400::Physics: 430en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Fysikk: 430en_US
dc.titleMulti-modal chip-based fluorescence and quantitative phase microscopy for studying inflammation in macrophagesen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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