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dc.contributor.authorKhider, Miriam
dc.contributor.authorWillassen, Nils Peder
dc.contributor.authorHansen, Hilde
dc.date.accessioned2019-03-28T11:58:37Z
dc.date.available2019-03-28T11:58:37Z
dc.date.issued2018-09-12
dc.description.abstract<i>Background</i>: Quorum sensing (QS) is a cell-to cell communication system that bacteria use to synchronize activities as a group. LitR, the master regulator of QS in <i>Aliivibrio salmonicida</i>, was recently shown to regulate activities such as motility, rugosity and biofilm formation in a temperature dependent manner. LitR was also found to be a positive regulator of <i>rpoQ</i>. RpoQ is an alternative sigma factor belonging to the sigma −70 family. Alternative sigma factors direct gene transcription in response to environmental signals. In this work we have studied the role of RpoQ in biofilm formation, colony morphology and motility of <i>A. salmonicida</i> LFI1238.<p> <p><i>Results</i>: The <i>rpoQ</i> gene in <i>A. salmonicida</i> LFI1238 was deleted using allelic exchange. We found that RpoQ is a strong repressor of rugose colony morphology and biofilm formation, and that it controls motility of the bacteria. We also show that overexpression of <i>rpoQ</i> in a <b>Δ</b><i>litR</i> mutant of <i>A. salmonicida</i> disrupts the biofilm produced by the <b>Δ</b><i>litR</i> mutant and decreases its motility, whereas <i>rpoQ</i> overexpression in the wild-type completely eliminates the motility.<p> <p><i>Conclusion</i>: The present work demonstrates that the RpoQ sigma factor is a novel regulatory component involved in modulating motility, colony morphology and biofilm formation in the fish pathogen <i>A. salmonicida</i>. The findings also confirm that RpoQ functions downstream of the QS master regulator LitR. However further studies are needed to elucidate how LitR and RpoQ work together in controlling phenotypes related to QS in <i>A. salmonicida</i>.en_US
dc.description.sponsorshipUiT The Arctic University of Norwayen_US
dc.descriptionSource at <a href=https://doi.org/10.1186/s12866-018-1258-9>https://doi.org/10.1186/s12866-018-1258-9. </a> © The Author(s). 2018en_US
dc.identifier.citationKhider, M., Willassen, N.P. & Hansen, H. (2018). The alternative sigma factor RpoQ regulates colony morphology, biofilm formation and motility in the fish pathogen <i>Aliivibrio salmonicida</i>. <i>BMC Microbiology</i>, 18:116. https://doi.org/10.1186/s12866-018-1258-9en_US
dc.identifier.cristinIDFRIDAID 1612548
dc.identifier.doi10.1186/s12866-018-1258-9
dc.identifier.issn1471-2180
dc.identifier.urihttps://hdl.handle.net/10037/15091
dc.language.isoengen_US
dc.publisherBMCen_US
dc.relation.ispartofKhider, M. (2019). Exploring Quorum Sensing Dynamics and Biofilm Formation in the Fish Pathogen <i>Aliivibrio salmonicida</i>. (Doctoral thesis). <a href=https://hdl.handle.net/10037/16612>https://hdl.handle.net/10037/16612</a>.
dc.relation.journalBMC Microbiology
dc.relation.urihttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-018-1258-9
dc.rights.accessRightsopenAccessen_US
dc.subjectAliivibrio salmonicidaen_US
dc.subjectSigma factorsen_US
dc.subjectRpoQen_US
dc.subjectTemperatureen_US
dc.subjectQuorum sensingen_US
dc.subjectMotilityen_US
dc.subjectBiofilmen_US
dc.subjectOverexpressionen_US
dc.subjectVDP::Mathematics and natural science: 400::Chemistry: 440en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Kjemi: 440en_US
dc.titleThe alternative sigma factor RpoQ regulates colony morphology, biofilm formation and motility in the fish pathogen Aliivibrio salmonicidaen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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