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dc.contributor.authorSalgado-Flores, Alejandro
dc.contributor.authorTveit, Alexander Tøsdal
dc.contributor.authorWright, A-D
dc.contributor.authorPope, Phillip
dc.contributor.authorSundset, Monica Alterskjær
dc.date.accessioned2019-06-06T12:05:46Z
dc.date.available2019-06-06T12:05:46Z
dc.date.issued2019-03-11
dc.description.abstractRock ptarmigans (<i>Lagopus muta</i>) are gallinaceous birds inhabiting arctic and sub-arctic environments. Their diet varies by season, including plants or plant parts of high nutritional value, but also toxic plant secondary metabolites (PSMs). Little is known about the microbes driving organic matter decomposition in the cecum of ptarmigans, especially the last steps leading to methanogenesis. The cecum microbiome in wild rock ptarmigans from Arctic Norway was characterized to unveil their functional potential for PSM detoxification, methanogenesis and polysaccharides degradation. Cecal samples were collected from wild ptarmigans from Svalbard (<i>L. m. hyperborea</i>) and northern Norway (<i>L. m. muta</i>) during autumn/winter (Sept-Dec). Samples from captive Svalbard ptarmigans fed commercial pelleted feed were included to investigate the effect of diet on microbial composition and function. Abundances of methanogens and bacteria were determined by qRT-PCR, while microbial community composition and functional potential were studied using 16S rRNA gene sequencing and shotgun metagenomics. Abundances of bacteria and methanogenic Archaea were higher in wild ptarmigans compared to captive birds. The ceca of wild ptarmigans housed bacterial groups involved in PSM-degradation, and genes mediating the conversion of phenol compounds to pyruvate. <i>Methanomassiliicoccaceae</i> was the major archaeal family in wild ptarmigans, carrying the genes for methanogenesis from methanol. It might be related to increased methanol production from pectin degradation in wild birds due to a diet consisting of primarily fresh pectin-rich plants. Both wild and captive ptarmigans possessed a broad suite of genes for the depolymerization of hemicellulose and non-cellulosic polysaccharides (e.g. starch). In conclusion, there were no physiological and phenotypical dissimilarities in the microbiota found in the cecum of wild ptarmigans on mainland Norway and Svalbard. While substantial differences in the functional potential for PSM degradation and methanogenesis in wild and captive birds seem to be a direct consequence of their dissimilar diets.en_US
dc.description.sponsorshipUiT The Arctic University of Norway Nansenfondet, 2014
dc.descriptionSource at <a href=https://doi.org/10.1371/journal.pone.0213503>https://doi.org/10.1371/journal.pone.0213503</a>.en_US
dc.identifier.citationSalgado-Flores, A., Tveit, A.T., Wright, A.-D., Pope, P.B. & Sundset, M.A. (2019). Characterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norway. <i>PLoS ONE, 14</i>(3), e0213503. https://doi.org/10.1371/journal.pone.0213503en_US
dc.identifier.issn1932-6203
dc.identifier.otherFRIDAID 1685450
dc.identifier.otherhttps://doi.org/10.1371/journal.pone.0213503
dc.identifier.urihttps://hdl.handle.net/10037/15475
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isbasedonThe sequence reads obtained from 16S amplicon sequencing and shotgun metagenomics are available at the <a href=https://www.ncbi.nlm.nih.gov/sra>Sequence Read Archive</a> (SRA) database under the BioProject identifier PRJNA450906.en_US
dc.relation.journalPLoS ONE
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRIBIO2/250479/Norway/Back to basics: simplifying microbial communities to decrypt complex interactions//
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRIMEDBIO/251027/Norway/ Time & Energy: Fundamental microbial mechanisms that control CH4 dynamics in a warming Arctic//
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/336355/EU/Interpreting the irrecoverable microbiota in digestive ecosystems/MICRODE/
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/608695/EU/ Marie Curie cofunding of the FRICON mobility programme in the Research Council of Norway scheme for independent basic research projects/FRICON/
dc.rights.accessRightsopenAccessen_US
dc.titleCharacterization of the cecum microbiome from wild and captive rock ptarmigans indigenous to Arctic Norwayen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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