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dc.contributor.authorDubey, Vishesh Kumar
dc.contributor.authorPopova, Daria
dc.contributor.authorAhmad, Azeem
dc.contributor.authorAcharya, Ganesh
dc.contributor.authorBasnet, Purusotam
dc.contributor.authorMehta, Dalip Singh
dc.contributor.authorAhluwalia, Balpreet Singh
dc.date.accessioned2020-01-23T07:05:38Z
dc.date.available2020-01-23T07:05:38Z
dc.date.issued2019-03-05
dc.description.abstractSemen quality assessed by sperm count and sperm cell characteristics such as morphology and motility, is considered to be the main determinant of men’s reproductive health. Therefore, sperm cell selection is vital in assisted reproductive technology (ART) used for the treatment of infertility. Conventional bright field optical microscopy is widely utilized for the imaging and selection of sperm cells based on the qualitative analysis by experienced clinicians. In this study, we report the development of a highly sensitive quantitative phase microscopy (QPM) using partially spatially coherent light source, which is a label-free, non-invasive and high-resolution technique to quantify various biophysical parameters. The partial spatial coherence nature of light source provides a significant improvement in spatial phase sensitivity and hence reconstruction of the phase of the entire sperm cell is demonstrated, which was otherwise not possible using highly spatially coherent light source. High sensitivity of the system enables quantitative phase imaging of the specimens having very low refractive index contrast with respect to the medium like tail of the sperm cells. Further, it also benefits with accurate quantification of 3D-morphological parameters of sperm cells which might be helpful in the infertility treatment. The quantitative analysis of more than 2500 sperm cells under hydrogen peroxide (H2O2) induced oxidative stress condition is demonstrated. It is further correlated with motility of sperm cell to study the effect of oxidative stress on healthy sperm cells. The results exhibit a decrease in the maximum phase values of the sperm head as well as decrease in the sperm cell’s motility with increasing oxidative stress, i.e., H2O2 concentration. Various morphological and texture parameters were extracted from the phase maps and subsequently support vector machine (SVM) based machine learning algorithm is employed for the classification of the control and the stressed sperms cells. The algorithm achieves an area under the receiver operator characteristic (ROC) curve of 89.93% based on the all morphological and texture parameters with a sensitivity of 91.18%. The proposed approach can be implemented for live sperm cells selection in ART procedure for the treatment of infertility.en_US
dc.identifier.citationDubey VK, Popova DA, Ahmad A, Acharya G, Basnet P, Mehta DS, Ahluwalia BS. Partially spatially coherent digital holographic microscopy and machine learning for quantitative analysis of human spermatozoa under oxidative stress condition. Scientific Reports. 2019;9(1)en_US
dc.identifier.cristinIDFRIDAID 1776879
dc.identifier.doihttps://doi.org/10.1038/s41598-019-39523-5
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10037/17194
dc.language.isoengen_US
dc.publisherNature Researchen_US
dc.relation.ispartofPopova, D. (2021). Advanced methods in reproductive medicine: Application of optical nanoscopy, artificial intelligence-assisted quantitative phase microscopy and mitochondrial DNA copy numbers to assess human sperm cells. (Doctoral thesis). <a href=https://hdl.handle.net/10037/22598>https://hdl.handle.net/10037/22598</a>.
dc.relation.journalScientific Reports
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700en_US
dc.subjectVDP::Medisinske Fag: 700en_US
dc.titlePartially spatially coherent digital holographic microscopy and machine learning for quantitative analysis of human spermatozoa under oxidative stress conditionen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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