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dc.contributor.authorShigeoka, Toshiaki
dc.contributor.authorKoppers, Max
dc.contributor.authorWong, Hovy Ho Wai
dc.contributor.authorLin, Julie Qiaojin
dc.contributor.authorCagnetta, Roberta
dc.contributor.authorDwivedy, Asha
dc.contributor.authorde Freitas Nascimento, Janaina
dc.contributor.authorvan Tartwijk, Francesca W.
dc.contributor.authorStröhl, Florian
dc.contributor.authorCioni, Jean Michel
dc.contributor.authorSchaeffer, Julia
dc.contributor.authorCarrington, Mark
dc.contributor.authorKaminski, Clemens F.
dc.contributor.authorJung, Hosung
dc.contributor.authorHarris, William A.
dc.contributor.authorHolt, Christine E.
dc.date.accessioned2020-01-28T12:36:26Z
dc.date.available2020-01-28T12:36:26Z
dc.date.issued2019-12-10
dc.description.abstractRibosome assembly occurs mainly in the nucleolus, yet recent studies have revealed robust enrichment and translation of mRNAs encoding many ribosomal proteins (RPs) in axons, far away from neuronal cell bodies. Here, we report a physical and functional interaction between locally synthesized RPs and ribosomes in the axon. We show that axonal RP translation is regulated through a sequence motif, CUIC, that forms an RNA-loop structure in the region immediately upstream of the initiation codon. Using imaging and subcellular proteomics techniques, we show that RPs synthesized in axons join axonal ribosomes in a nucleolus-independent fashion. Inhibition of axonal CUIC-regulated RP translation decreases local translation activity and reduces axon branching in the developing brain, revealing the physiological relevance of axonal RP synthesis in vivo. These results suggest that axonal translation supplies cytoplasmic RPs to maintain/modify local ribosomal function far from the nucleolus in neurons.en_US
dc.identifier.citationShigeoka,T., Koppers, M., Wong, H., Lin, J.Q., Cagnetta, R., Dwivedy, A., ...Holt, C.E. (2019) On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.<i> Cell reports, 29</i>, 3605-3619.en_US
dc.identifier.cristinIDFRIDAID 1781854
dc.identifier.doi10.1016/j.celrep.2019.11.025
dc.identifier.issn2211-1247
dc.identifier.urihttps://hdl.handle.net/10037/17245
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalCell reports
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Author(s)en_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Cell biology: 471en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Cellebiologi: 471en_US
dc.titleOn-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axonsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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