dc.contributor.author | Opstad, Ida Sundvor | |
dc.contributor.author | Ströhl, Florian | |
dc.contributor.author | Birgisdottir, Åsa birna | |
dc.contributor.author | Acuña Maldonado, Sebastian Andres | |
dc.contributor.author | Kalstad, Trine | |
dc.contributor.author | Myrmel, Truls | |
dc.contributor.author | Agarwal, Krishna | |
dc.contributor.author | Ahluwalia, Balpreet Singh | |
dc.date.accessioned | 2020-03-16T08:07:43Z | |
dc.date.available | 2020-03-16T08:07:43Z | |
dc.date.issued | 2019-07-22 | |
dc.description.abstract | In this work we have explored the live-cell friendly nanoscopy method Multiple Signal Classification Algorithm (MUSICAL) for multi-colour imaging of various organelles and sub-cellular structures in the cardiomyoblast cell line H2c9. We have tested MUSICAL for fast (up to 230Hz), multi-colour time-lapse sequences of various sub-cellular structures (mitochondria, endoplasmic reticulum, microtubules, endosomes and nuclei) in living cells using low excitation-light dose. Challenges and opportunities with applying MUSICAL for studying rapid sub-cellular dynamics are discussed. | en_US |
dc.identifier.citation | Opstad, I.S., Ströhl, F., Birgisdottir, Å.b., Maldonado, S.A.A., Kalstad, T., Myrmel, T., Agarwal, K., Ahluwalia, B.S. (2019) Adaptive fluctuation imaging captures rapid subcellular dynamics. <i> Proceedings of SPIE, the International Society for Optical Engineering, Advances in Microscopic Imaging II, 11076,1-3 </i> | en_US |
dc.identifier.cristinID | FRIDAID 1798701 | |
dc.identifier.doi | 10.1117/12.2526846 | |
dc.identifier.issn | 1605-7422 | |
dc.identifier.issn | 1042-4687 | |
dc.identifier.uri | https://hdl.handle.net/10037/17740 | |
dc.language.iso | eng | en_US |
dc.publisher | Society of Photo-Optical Instrumentation Engineers (SPIE) | en_US |
dc.relation.ispartof | Opstad, I.S. (2021). Bringing optical nanoscopy to life - Super-resolution microscopy of living cells. (Doctoral thesis). <a href=https://hdl.handle.net/10037/20306>https://hdl.handle.net/10037/20306</a> | |
dc.relation.journal | Progress in Biomedical Optics and Imaging | |
dc.rights.accessRights | openAccess | en_US |
dc.rights.holder | © SPIE. (2019) Society of Photo-Optical Instrumentation Engineers, SPIE | en_US |
dc.subject | VDP::Technology: 500 | en_US |
dc.subject | VDP::Teknologi: 500 | en_US |
dc.title | Adaptive fluctuation imaging captures rapid subcellular dynamics | en_US |
dc.type.version | submittedVersion | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |