• Engineering low-temperature expression systems for heterologous production of cold-adapted enzymes 

      Bjerga, Gro Elin Kjæreng; Lale, Rahmi; Williamson, Adele Kim (Journal article; Tidsskriftartikkel; Peer reviewed, 2016-02-26)
      Production of psychrophilic enzymes in the commonly used mesophilic expression systems is hampered by low intrinsic stability of the recombinant enzymes at the optimal host growth temperatures. Unless strategies for low-temperature expression are advanced, research on psychrophilic enzymes may end up being biased toward those that can be stably produced in commonly used mesophilic host systems. Two ...
    • Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens 

      Tietze, Lisa; Hoff, Maria Wilhelmsen; Lale, Rahmi (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-01-27)
      Vibrio natriegens has recently gained attention as a novel fast-growing bacterium in synthetic biology applications. Currently, a limited set of genetic elements optimised for Escherichia coli are used in V. natriegens due to the lack of DNA parts characterised in this novel host. In this study, we report the identification and cross-characterisation of artificial promoters and 5′ untranslated ...