Browsing Artikler, rapporter og annet (kjemi) by Author "Larsen, Atle Noralf"
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Characterization and engineering of a DNA polymerase reveals a single amino-acid substitution in the fingers subdomain to increase strand-displacement activity of A-family prokaryotic DNA polymerases
Piotrowski, Yvonne; Gurung, Man Kumari; Larsen, Atle Noralf (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-08-09)<i>Background</i> - The discovery of thermostable DNA polymerases such as Taq DNA polymerase revolutionized amplification of DNA by polymerase chain reaction methods that rely on thermal cycling for strand separation. These methods are widely used in the laboratory for medical research, clinical diagnostics, criminal forensics and general molecular biology research. Today there is a growing demand ... -
Characterization of an intertidal zone metagenome oligoribonuclease and the role of the intermolecular disulfide bond for homodimer formation and nuclease activity
Piotrowski, Yvonne; Berg, Kristel; Klebl, David Paul; Leiros, Ingar; Larsen, Atle Noralf (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-08-17)The gene encoding MG Orn has been identified from a metagenomic library created from the intertidal zone in Svalbard and encodes a protein of 184 amino acid residues. The <i>mg orn</i> gene has been cloned, recombinantly expressed in <i>Escherichia coli</i>, and purified to homogeneity. Biochemical characterization of the enzyme showed that it efficiently degrades short RNA oligonucleotide substrates ...