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dc.contributor.authorKrause, Kirsten
dc.contributor.authorOlsen, Stian
dc.date.accessioned2021-01-25T12:07:48Z
dc.date.available2021-01-25T12:07:48Z
dc.date.issued2020-07-03
dc.description.abstractCellulolytic activity can be measured using a variety of methods, the choice of which depends on the raw material and goals. An inexpensive, rapid, and reliable method, suitable for plants and other sources alike, is based on digestion of the easily degradable soluble cellulose derivative carboxymethylcellulose (CMC). Direct detection of CMC digestion by cellulolytic activity is based on the “negative staining principle,” where undigested CMC is stained with appropriate colorimetric or fluorescent stains, while CMC exposed to digestion by cellulase shows a reduction in staining intensity. The reduction is proportional to the enzyme activity and is not influenced by endogenous levels of glucose in the sample, making this method applicable for a wide variety of samples, including plant material.en_US
dc.descriptionThis is a post-peer-review, pre-copyedit version of an article published in <i>The Plant Cell Wall Methods and Protocols</i>. The final authenticated version is available online at: <a href=https://doi.org/10.1007/978-1-0716-0621-6>https://doi.org/10.1007/978-1-0716-0621-6. </a>en_US
dc.identifier.citationKrause K, Olsen S. Screening for Cellulolytic Plant Enzymes Using Colorimetric and Fluorescence Methods. Methods in molecular biology. 2020;2145:193-201en_US
dc.identifier.cristinIDFRIDAID 1844705
dc.identifier.doi10.1007/978-1-0716-0621-6_11
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.urihttps://hdl.handle.net/10037/20466
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.relation.ispartofseriesMethods in Molecular Biology book series (MIMB, volume 2149)en_US
dc.relation.journalMethods in molecular biology
dc.relation.projectIDTromsø forskningsstiftelse: TFS Toppforsk 16-TF-KKen_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.titleScreening for Cellulolytic Plant Enzymes Using Colorimetric and Fluorescence Methodsen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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