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dc.contributor.authorAhmad, Azeem
dc.contributor.authorDubey, Vishesh
dc.contributor.authorJayakumar, Nikhil
dc.contributor.authorHabib, Anowarul
dc.contributor.authorButola, Ankit
dc.contributor.authorNystad, Mona
dc.contributor.authorAcharya, Ganesh
dc.contributor.authorBasnet, Purusotam
dc.contributor.authorMehta, Dalip Singh
dc.contributor.authorAhluwalia, Balpreet Singh
dc.date.accessioned2021-11-18T10:16:11Z
dc.date.available2021-11-18T10:16:11Z
dc.date.issued2021-08-04
dc.description.abstractHigh space-bandwidth product with high spatial phase sensitivity is indispensable for a single-shot quantitative phase microscopy (QPM) system. It opens avenue for widespread applications of QPM in the field of biomedical imaging. Temporally low coherence light sources are implemented to achieve high spatial phase sensitivity in QPM at the cost of either reduced temporal resolution or smaller field of view (FOV). In addition, such light sources have low photon degeneracy. On the contrary, high temporal coherence light sources like lasers are capable of exploiting the full FOV of the QPM systems at the expense of less spatial phase sensitivity. In the present work, we demonstrated that use of narrowband partially spatially coherent light source also called pseudo-thermal light source (PTLS) in QPM overcomes the limitations of conventional light sources. The performance of PTLS is compared with conventional light sources in terms of space bandwidth product, phase sensitivity and optical imaging quality. The capabilities of PTLS are demonstrated on both amplitude (USAF resolution chart) and phase (thin optical waveguide, height ~ 8 nm) objects. The spatial phase sensitivity of QPM using PTLS is measured to be equivalent to that for white light source and supports the FOV (18 times more) equivalent to that of laser light source. The high-speed capabilities of PTLS based QPM is demonstrated by imaging live sperm cells that is limited by the camera speed and large FOV is demonstrated by imaging histopathology human placenta tissue samples. Minimal invasive, high-throughput, spatially sensitive and single-shot QPM based on PTLS will enable wider penetration of QPM in life sciences and clinical applications.en_US
dc.identifier.citationAhmad, Dubey, Jayakumar, Habib, Butola, Nystad, Acharya, Basnet, Mehta, Ahluwalia. High-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination. Scientific Reports. 2021;11(1):1-13en_US
dc.identifier.cristinIDFRIDAID 1946663
dc.identifier.doi10.1038/s41598-021-94915-w
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10037/23066
dc.language.isoengen_US
dc.publisherNature Researchen_US
dc.relation.journalScientific Reports
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/NANO2021/288565/Norway/Integrated photonic chip-based nanoscopy for pathology & the clinic//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/BIOTEK2021/285571/Norway/Optimalisering: High-throughput and high-resolution pathology using chip-based nanoscopy//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Mathematics and natural science: 400::Physics: 430en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Fysikk: 430en_US
dc.titleHigh-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illuminationen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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