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dc.contributor.authorSunde, Marianne
dc.contributor.authorRamstad, Silje N.
dc.contributor.authorRudi, Knut
dc.contributor.authorPorcellato, Davide
dc.contributor.authorRavi, Anuradha
dc.contributor.authorLudvigsen, Jane
dc.contributor.authordas Neves, Carlos Gonçalo
dc.contributor.authorTryland, Morten
dc.contributor.authorRopstad, Erik
dc.contributor.authorSlettemeås, Jannice S.
dc.contributor.authorTelke, Amar A.
dc.date.accessioned2021-11-19T08:34:27Z
dc.date.available2021-11-19T08:34:27Z
dc.date.issued2021-06-30
dc.description.abstractObjectives - In extreme environments, such as the Arctic region, the anthropogenic influence is low and the presence of antimicrobial-resistant bacteria is unexpected. In this study, we screened wild reindeer (Rangifer tarandus platyrhynchus) from the Svalbard High Arctic Archipelago for antimicrobial-resistant Escherichia coli and performed in-depth strain characterisation.<p> <p>Methods - Using selective culturing of faecal samples from 55 animals, resistant E. coli were isolated and subjected to minimum inhibitory concentration (MIC) determination, conjugation experiments and whole-genome sequencing.<p> <p>Results - Twelve animals carried antimicrobial-resistant E. coli. Genomic analysis showed IncF plasmids as vectors both for resistance and virulence genes in most strains. Plasmid-associated genes encoding resistance to ampicillin, sulfonamides, streptomycin and trimethoprim were found in addition to virulence genes typical for colicin V (ColV)-producing plasmids. Comparison with previously reported IncF ColV plasmids from human and animal hosts showed high genetic similarity. The plasmids were detected in E. coli sequence types (STs) previously described as hosts for such plasmids, such as ST58, ST88 and ST131.<p> <p>Conclusion - Antimicrobial-resistant E. coli were detected from Svalbard reindeer. Our findings show that successful hybrid antimicrobial resistance–ColV plasmids and their host strains are widely distributed also occurring in extreme environmental niches such as arctic ecosystems. Possible introduction routes of resistant bacterial strains and plasmids into Svalbard ecosystems may be through migrating birds, marine fish or mammals, arctic fox (Vulpes lagopus) or via human anthropogenic activities such as tourism.en_US
dc.identifier.citationSunde, Ramstad, Rudi, Porcellato, Ravi, Ludvigsen, das Neves CG, Tryland, Ropstad, Slettemeås, Telke. Plasmid-associated antimicrobial resistance and virulence genes in Escherichia coli in a high arctic reindeer subspecies. Journal of Global Antimicrobial Resistance. 2021;26:317-322en_US
dc.identifier.cristinIDFRIDAID 1947806
dc.identifier.doi10.1016/j.jgar.2021.06.003
dc.identifier.issn2213-7165
dc.identifier.issn2213-7173
dc.identifier.urihttps://hdl.handle.net/10037/23076
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalJournal of Global Antimicrobial Resistance
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.titlePlasmid-associated antimicrobial resistance and virulence genes in Escherichia coli in a high arctic reindeer subspeciesen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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