dc.contributor.author | Acuna Maldonado, Sebastian Andres | |
dc.contributor.author | ROY, MAYANK | |
dc.contributor.author | Villegas, Luis | |
dc.contributor.author | Dubey, Vishesh Kumar | |
dc.contributor.author | Ahluwalia, Balpreet Singh | |
dc.contributor.author | Agarwal, Krishna | |
dc.date.accessioned | 2022-01-31T12:06:43Z | |
dc.date.available | 2022-01-31T12:06:43Z | |
dc.date.issued | 2021-08-11 | |
dc.description.abstract | Contrast in fluorescence microscopy images allows for the differentiation between different structures by their difference in intensities. However, factors such as point-spread function and noise may reduce it, affecting its interpretability. We identified that fluctuation of emitters in a stack of images can be exploited to achieve increased contrast when compared to the average and Richardson-Lucy deconvolution. We tested our methods on four increasingly challenging samples including tissue, in which case results were comparable to the ones obtained by structured illumination microscopy in terms of contrast. | en_US |
dc.identifier.citation | Acuna Maldonado, ROY, Villegas, Dubey, Ahluwalia, Agarwal. Deriving high contrast fluorescence microscopy images through low contrast noisy image stacks. Biomedical Optics Express. 2021 | en_US |
dc.identifier.cristinID | FRIDAID 1985211 | |
dc.identifier.doi | 10.1364/BOE.422747 | |
dc.identifier.issn | 2156-7085 | |
dc.identifier.uri | https://hdl.handle.net/10037/23845 | |
dc.language.iso | eng | en_US |
dc.publisher | Optical Society of America | en_US |
dc.relation.ispartof | Acuna Maldonado, S.A. (2023). Multiple Signal Classification Algorithm: A computational microscopy tool for fluorescence microscopy. (Doctoral thesis). <a href=https://hdl.handle.net/10037/31879>https://hdl.handle.net/10037/31879</a>. | |
dc.relation.journal | Biomedical Optics Express | |
dc.relation.projectID | info:eu-repo/grantAgreement/RCN/FRINATEK/288082/Norway/Label-free nanoscopy of living cells through nanoscale refractive index profiler// | en_US |
dc.relation.projectID | info:eu-repo/grantAgreement/RCN/?/804233?/Norway/?/?/ | en_US |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/749666/Norway/Chip-based MUSICAL nanoscopy for imaging endocytosis pathways of phage viruses in liver sinusoidal endothelial cells/MUSICAL/ | en_US |
dc.rights.accessRights | openAccess | en_US |
dc.rights.holder | Copyright 2021 The Author(s) | en_US |
dc.title | Deriving high contrast fluorescence microscopy images through low contrast noisy image stacks | en_US |
dc.type.version | publishedVersion | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |
dc.type | Peer reviewed | en_US |