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dc.contributor.authorButola, Ankit
dc.contributor.authorCoucheron, David Andre
dc.contributor.authorSzafranska, Karolina
dc.contributor.authorAhmad, Azeem
dc.contributor.authorMao, Hong
dc.contributor.authorTinguely, Jean-Claude
dc.contributor.authorMcCourt, Peter Anthony
dc.contributor.authorParamasivam, Senthilkumaran
dc.contributor.authorMehta, Dalip Singh
dc.contributor.authorAgarwal, Krishna
dc.contributor.authorAhluwalia, Balpreet Singh
dc.date.accessioned2022-02-24T13:11:18Z
dc.date.available2022-02-24T13:11:18Z
dc.date.issued2021-11-23
dc.description.abstractVisualization of three-dimensional (3D) morphological changes in the subcellular structures of a biological specimen is a major challenge in life science. Here, we present an integrated chip-based optical nanoscopy combined with quantitative phase microscopy (QPM) to obtain 3D morphology of liver sinusoidal endothelial cells (LSEC). LSEC have unique morphology with small nanopores (50-300 nm in diameter) in the plasma membrane, called fenestrations. The fenestrations are grouped in discrete clusters, which are around 100 to 200 nm thick. Thus, imaging and quantification of fenestrations and sieve plate thickness require resolution and sensitivity of sub-100 nm along both the lateral and the axial directions, respectively. In chip-based nanoscopy, the optical waveguides are used both for hosting and illuminating the sample. The fluorescence signal is captured by an upright microscope, which is converted into a Linnik-type interferometer to sequentially acquire both superresolved images and phase information of the sample. The multimodal microscope provided an estimate of the fenestration diameter of 119 +/- 53 nm and average thickness of the sieve plates of 136.6 +/- 42.4 nm, assuming the constant refractive index of cell membrane to be 1.38. Further, LSEC were treated with cytochalasin B to demonstrate the possibility of precise detection in the cell height. The mean phase value of the fenestrated area in normal and treated cells was found to be 161 +/- 50 mrad and 109 +/- 49 mrad, respectively. The proposed multimodal technique offers nanoscale visualization of both the lateral size and the thickness map, which would be of broader interest in the fields of cell biology and bioimaging.en_US
dc.identifier.citationButola A, Coucheron DA, Szafranska KJ, Ahmad A, Mao H, Tinguely J, McCourt PAG, Paramasivam S, Mehta DS, Agarwal K, Ahluwalia BS. Multimodal on-chip nanoscopy and quantitative phase imaging reveals the nanoscale morphology of liver sinusoidal endothelial cells. Proceedings of the National Academy of Sciences of the United States of America. 2021;118(47)en_US
dc.identifier.cristinIDFRIDAID 1982378
dc.identifier.doi10.1073/pnas.2115323118
dc.identifier.issn0027-8424
dc.identifier.issn1091-6490
dc.identifier.urihttps://hdl.handle.net/10037/24132
dc.language.isoengen_US
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/766181/Germany/Super-resolution optical microscopy of nanosized pore dynamics in endothelial cells/DeLIVER/en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/804233/Norway/Label-free 3D morphological nanoscopy for studying sub-cellular dynamics in live cancer cells with high spatio-temporal resolution/3D-nanoMorph/en_US
dc.relation.urihttps://www.pnas.org/content/pnas/118/47/e2115323118.full.pdf
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.titleMultimodal on-chip nanoscopy and quantitative phase imaging reveals the nanoscale morphology of liver sinusoidal endothelial cellsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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