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dc.contributor.authorLaulund, Anne Sofie
dc.contributor.authorSchwartz, Franziska Angelika
dc.contributor.authorChristophersen, Lars
dc.contributor.authorHøiby, Niels
dc.contributor.authorSvendsen, John Sigurd Mjøen
dc.contributor.authorStensen, Wenche Gunvor Berg
dc.contributor.authorThomsen, Kim
dc.contributor.authorCavanagh, Jorunn Pauline
dc.contributor.authorMoser, Claus
dc.date.accessioned2022-05-16T07:37:59Z
dc.date.available2022-05-16T07:37:59Z
dc.date.issued2021-12-23
dc.description.abstractObjectives: Chronic wounds are characterised by prolonged inflammation, low mitogenic activity, high protease/low inhibitor activity, microbiota changes and biofilm formation, combined with the aetiology of the original insult. One strategy to promote healing is to terminate the parasitism-like relationship between the biofilm-growing pathogen and host response. Antimicrobial peptide AMC-109 is a potential treatment with low resistance potential and broad-spectrum coverage with rapid bactericidal effect. We aimed to investigate whether adjunctive AMC-109 could augment the ciprofloxacin effect in a chronic Pseudomonas aeruginosa wound model.<p> <p>Methods: Third-degree burns were inflicted on 33 BALB/c mice. Pseudomonas aeruginosa embedded in seaweed alginate was injected sub-eschar to mimic biofilm. Mice were randomised to receive AMC-109, combined AMC-109 and ciprofloxacin, ciprofloxacin, or placebo for 5 days followed by sample collection.<p> Results: A lower bacterial load was seen in the double-treated group compared with either monotherapy group (AMC-109, p = 0.0076; ciprofloxacin, p = 0.0266). To evaluate the innate host response, cytokines and growth factors were quantified. The pro-inflammatory response was dampened in the double-treated mice compared with the mono-ciprofloxacin-treated group (p = 0.0009). Lower mobilisation of neutrophils from the bone marrow was indicated by reduced G-CSF in all treatment groups compared with placebo. Improved tissue remodelling was indicated by the highest level of tissue inhibitor of metalloproteases and low metalloprotease level in the double-treated group.<p> Conclusion: AMC-109 showed adjunctive antipseudomonal abilities augmenting the antimicrobial effect of ciprofloxacin in this wound model. The study indicates a potential role for AMC-109 in treating chronic wounds with complicating biofilm infections.en_US
dc.identifier.citationLaulund, Schwartz, Christophersen, Høiby, Svendsen, Stensen, Thomsen, Cavanagh, Moser. Lactoferricin-inspired peptide AMC-109 augments the effect of ciprofloxacin against Pseudomonas aeruginosa biofilm in chronic murine wounds. Journal of Global Antimicrobial Resistance. 2022en_US
dc.identifier.cristinIDFRIDAID 2020804
dc.identifier.doi10.1016/j.jgar.2021.12.015
dc.identifier.issn2213-7165
dc.identifier.issn2213-7173
dc.identifier.urihttps://hdl.handle.net/10037/25135
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalJournal of Global Antimicrobial Resistance
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.titleLactoferricin-inspired peptide AMC-109 augments the effect of ciprofloxacin against Pseudomonas aeruginosa biofilm in chronic murine woundsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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