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dc.contributor.authorSarre, Aili
dc.contributor.authorStelter, Meike
dc.contributor.authorRollo, Filipe
dc.contributor.authorDe Bonis, Salvatore
dc.contributor.authorSeck, Anna
dc.contributor.authorHognon, Cecilia
dc.contributor.authorRavanat, Jean-Luc
dc.contributor.authorMonari, Antonio
dc.contributor.authorDehez, Francois
dc.contributor.authorMoe, Elin
dc.contributor.authorTimmins, Joanna
dc.date.accessioned2022-05-19T08:40:59Z
dc.date.available2022-05-19T08:40:59Z
dc.date.issued2019-03-28
dc.description.abstractEndonuclease III (EndoIII) is a bifunctional DNA glycosylase that removes oxidized pyrimidines from DNA. The genome of Deinococcus radiodurans encodes for an unusually high number of DNA glycosylases, including three EndoIII enzymes (drEndoIII1-3). Here, we compare the properties of these enzymes to those of their well-studied homologues from E. coli and human. Our biochemical and mutational data, reinforced by MD simulations of EndoIII-DNA complexes, reveal that drEndoIII2 exhibits a broad substrate specificity and a catalytic efficiency surpassing that of its counterparts. In contrast, drEndoIII1 has much weaker and uncoupled DNA glycosylase and AP-lyase activities, a characteristic feature of eukaryotic DNA glycosylases, and was found to present a relatively robust activity on single-stranded DNA substrates. To our knowledge, this is the first report of such an activity for an EndoIII. In the case of drEndoIII3, no catalytic activity could be detected, but its ability to specifically recognize lesion-containing DNA using a largely rearranged substrate binding pocket suggests that it may play an alternative role in genome maintenance. Overall, these findings reveal that D. radiodurans possesses a unique set of DNA repair enzymes, including three non-redundant EndoIII variants with distinct properties and complementary activities, which together contribute to genome maintenance in this bacterium.en_US
dc.identifier.citationSarre A, Stelter, Rollo, De Bonis, Seck, Hognon, Ravanat, Monari A, Dehez, Moe E, Timmins J. The three Endonuclease III variants of Deinococcus radiodurans possess distinct and complementary DNA repair activities. DNA Repair. 2019;78:45-59en_US
dc.identifier.cristinIDFRIDAID 1742344
dc.identifier.doi10.1016/j.dnarep.2019.03.014
dc.identifier.issn1568-7864
dc.identifier.issn1568-7856
dc.identifier.urihttps://hdl.handle.net/10037/25220
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalDNA Repair
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Author(s)en_US
dc.titleThe three Endonuclease III variants of Deinococcus radiodurans possess distinct and complementary DNA repair activitiesen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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