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dc.contributor.authorLomet, Didier
dc.contributor.authorDruart, Xavier
dc.contributor.authorHazlerigg, David
dc.contributor.authorBeltramo, Massimiliano
dc.contributor.authorDardente, Hugues
dc.date.accessioned2022-06-02T12:03:20Z
dc.date.available2022-06-02T12:03:20Z
dc.date.issued2020-05-15
dc.description.abstractThyroid hormone (TH) and estradiol (E2) direct seasonal switches in ovine reproductive physiology. In sheep, as in other mammals and birds, control of thyrotropin (TSH) production by the pars tuberalis (PT) links photoperiod responsiveness to seasonal breeding. PT-derived TSH governs opposite seasonal patterns of the TH deiodinases Dio2/Dio3 expression in tanycytes of the neighboring medio-basal hypothalamus (MBH), which explain the key role of TH. We recently used RNA-Seq to identify seasonal markers in the MBH and define the impact of TH. This impact was found to be quite limited, in terms of number of target genes, and very restricted with regards to neuroanatomical location, as TH specifically impacts genes expressed in tanycytes and hypothalamus, not in the PT. Here we address the impact of E2 on these seasonal markers, which are specifically expressed in either PT, tanycytes or hypothalamus. We also investigate if progesterone (P4) may be involved in timing the seasonal transition to anestrus. Our analysis provides circuit-level insights into the impact of sex steroids on the ewe seasonal breeding cycle. First, seasonal gene expression in the PT is independent of the sex steroid status. The fact that seasonal gene expression in the PT is also TH-independent strengthens the view that the PT is a circannual timer. Second, select tanycytic markers display some level of responsiveness to E2 and P4, which indicates another potential level of feedback control by sex steroids. Third, Kiss1 neurons of the arcuate nucleus are responsive to both TH and E2, which places them at the crossroads of photoperiodic transduction pathway and sex steroid feedback. This provides strong support to the concept that these Kiss1 neurons are pivotal to the long-recognized “seasonal switch in the ability of E2 to exert negative feedback”, which drives seasonal breeding.en_US
dc.descriptionAccepted manuscript version, licensed <a href=http://creativecommons.org/licenses/by-nc-nd/4.0/> CC BY-NC-ND 4.0. </a>en_US
dc.identifier.citationLomet, Druart, Hazlerigg, Beltramo, Dardente. Circuit-level analysis identifies target genes of sex steroids in ewe seasonal breeding. Molecular and Cellular Endocrinology. 2020;512en_US
dc.identifier.cristinIDFRIDAID 1886451
dc.identifier.doi10.1016/j.mce.2020.110825
dc.identifier.issn0303-7207
dc.identifier.issn1872-8057
dc.identifier.urihttps://hdl.handle.net/10037/25356
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalMolecular and Cellular Endocrinology
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7-PEOPLE /320553/EU/Deciphering the role of thyroid hormones in seasonal reproduction /THYROREPRO/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 Elsevieren_US
dc.titleCircuit-level analysis identifies target genes of sex steroids in ewe seasonal breedingen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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