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Chip-based multimodal super-resolution microscopy for histological investigations of cryopreserved tissue sections

Permanent link
https://hdl.handle.net/10037/26662
DOI
https://doi.org/10.1038/s41377-022-00731-w
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Date
2022-02-24
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Author
Villegas, Luis; Dubey, Vishesh Kumar; Nystad, Mona; Tinguely, Jean-Claude; Coucheron, David Andre; Dullo, Firehun Tsige; Priyadarshi, Anish; Acuna Maldonado, Sebastian Andres; Ahmad, Azeem; Mateos, Jose M.; Barmettler, Gery; Ziegler, Urs; Birgisdottir, Åsa Birna; Hovd, Aud-Malin Karlsson; Fenton, Kristin Andreassen; Acharya, Ganesh; Agarwal, Krishna; Ahluwalia, Balpreet Singh
Abstract
Histology involves the observation of structural features in tissues using a microscope. While diffraction-limited optical microscopes are commonly used in histological investigations, their resolving capabilities are insufficient to visualize details at subcellular level. Although a novel set of super-resolution optical microscopy techniques can fulfill the resolution demands in such cases, the system complexity, high operating cost, lack of multi-modality, and lowthroughput imaging of these methods limit their wide adoption for histological analysis. In this study, we introduce the photonic chip as a feasible high-throughput microscopy platform for super-resolution imaging of histological samples. Using cryopreserved ultrathin tissue sections of human placenta, mouse kidney, pig heart, and zebrafish eye retina prepared by the Tokuyasu method, we demonstrate diverse imaging capabilities of the photonic chip including total internal reflection fluorescence microscopy, intensity fluctuation-based optical nanoscopy, single-molecule localization microscopy, and correlative light-electron microscopy. Our results validate the photonic chip as a feasible imaging platform for tissue sections and pave the way for the adoption of super-resolution high-throughput multimodal analysis of cryopreserved tissue samples both in research and clinical settings.
Is part of
Villegas-Hernández, L.E. (2023). Super-resolution histology. (Doctoral thesis). https://hdl.handle.net/10037/31846.
Publisher
Nature
Citation
Villegas-Hernández, L.E., Dubey, V., Nystad, M. et al. Chip-based multimodal super-resolution microscopy for histological investigations of cryopreserved tissue sections. Light Sci Appl 11, 43 (2022)
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