dc.contributor.author | Ahmad, Azeem | |
dc.contributor.author | Jayakumar, Nikhil | |
dc.contributor.author | Ahluwalia, Balpreet Singh | |
dc.date.accessioned | 2022-11-14T09:22:04Z | |
dc.date.available | 2022-11-14T09:22:04Z | |
dc.date.issued | 2022-06-27 | |
dc.description.abstract | Dynamic speckle illumination (DSI) has recently attracted strong attention in the feld of biomedical
imaging as it pushes the limits of interference microscopy (IM) in terms of phase sensitivity, and
spatial and temporal resolution compared to conventional light source illumination. To date, despite
conspicuous advantages, it has not been extensively implemented in the feld of phase imaging
due to inadequate understanding of interference fringe formation, which is challenging to obtain in
dynamic speckle illumination interference microscopy (DSI-IM). The present article provides the basic
understanding of DSI through both simulation and experiments that is essential to build interference
microscopy systems such as quantitative phase microscopy, digital holographic microscopy and
optical coherence tomography. Using the developed understanding of DSI, we demonstrated its
capabilities which enables the use of non-identical objective lenses in both arms of the interferometer
and opens the fexibility to use user-defned microscope objective lens for scalable feld of view
and resolution phase imaging. It is contrary to the present understanding which forces us to use
identical objective lenses in conventional IM system and limits the applicability of the system for
fxed objective lens. In addition, it is also demonstrated that the interference fringes are not washed
out over a large range of optical path diference (OPD) between the object and the reference arm
providing competitive edge over low temporal coherence light source based IM system. The theory
and explanation developed here would enable wider penetration of DSI-IM for applications in biology
and material sciences. | en_US |
dc.identifier.citation | Ahmad, Jayakumar, Ahluwalia. Demystifying speckle field interference microscopy. Scientific Reports. 2022;12(1) | en_US |
dc.identifier.cristinID | FRIDAID 2054529 | |
dc.identifier.doi | 10.1038/s41598-022-14739-0 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://hdl.handle.net/10037/27352 | |
dc.language.iso | eng | en_US |
dc.publisher | Springer Nature | en_US |
dc.relation.journal | Scientific Reports | |
dc.rights.accessRights | openAccess | en_US |
dc.rights.holder | Copyright 2022 The Author(s) | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | en_US |
dc.rights | Attribution 4.0 International (CC BY 4.0) | en_US |
dc.title | Demystifying speckle field interference microscopy | en_US |
dc.type.version | publishedVersion | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |
dc.type | Peer reviewed | en_US |