Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure
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https://hdl.handle.net/10037/31040Date
2023-08-17Type
Journal articleTidsskriftartikkel
Peer reviewed
Author
Mao, Hong; Szafranska, Karolina; Kruse, Larissa Dorothea; Holte, Christopher Florian; Wolfson, Deanna; Ahluwalia, Balpreet Singh; Whitchurch, Cynthia B.; Cole, Louise; Lockwood, Glen P.; Diekmann, Robin; Le Couteur, David; Cogger, Victoria C.; McCourt, Peter AnthonyAbstract
Xanthines such as caffeine and theobromine are among the most consumed psychoactive stimulants
in the world, either as natural components of cofee, tea and chocolate, or as added ingredients. The
present study assessed if xanthines affect liver sinusoidal endothelial cells (LSEC). Cultured primary rat
LSEC were challenged with xanthines at concentrations typically obtained from normal consumption
of xanthine-containing beverages, food or medicines; and at higher concentrations below the in vitro
toxic limit. The fenestrated morphology of LSEC were examined with scanning electron and structured
illumination microscopy. All xanthine challenges had no toxic effects on LSEC ultrastructure as judged
by LSEC fenestration morphology, or function as determined by endocytosis studies. All xanthines
in high concentrations (150 μg/mL) increased fenestration frequency but at physiologically relevant
concentrations, only theobromine (8 μg/mL) showed an effect. LSEC porosity was influenced only by
high caffeine doses which also shifted the fenestration distribution towards smaller pores. Moreover,
a dose-dependent increase in fenestration number was observed after caffeine treatment. If these
compounds induce similar changes in vivo, age-related reduction of LSEC porosity can be reversed by
oral treatment with theobromine or with other xanthines using targeted delivery.
Publisher
Springer NatureCitation
Mao, Szafranska, Kruse, Holte, Wolfson, Ahluwalia, Whitchurch, Cole, Lockwood, Diekmann, Le Couteur, Cogger, McCourt. Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure. Scientific Reports. 2023;13(1)Metadata
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